河北医学2025，Vol.31Issue(11)：1787-1793,7.DOI:10.3969/j.issn.1006-6233.2025.11.05
黄芩苷调控cAMP/CREB/BDNF通路对自闭症大鼠自闭样行为和神经元损伤的影响
Effects of Baicalein Modulation of the cAMP/CREB/BDNF Pathway on Autistic-Like Behaviour and Neuronal Damage in Autistic Rats
摘要
Abstract
Objective:To explore the effects of baicalin on autistic-like behavior and neuronal damage in rats with autism by regulating cyclic adenosine monophosphate(cAMP)/cyclic adenosine monophosphate response element binding protein(CREB)/brain-derived neurotrophic factor(BDNF)pathway.Methods:A rat model of autism was established by intraperitoneal injection of valproic acid during pregnancy.The off-spring were assigned into model group,baicalin group(100mg/kg),and baicalin+cAMP inhibitor(SQ22536)group(100mg/kg baicalin+2.22mg/kg SQ22536),with 12 rats in each group.The offspring of normal rats were considered as control group.The open field experiment was used to evaluate the spatial cogni-tion and exploration ability of rats.Three-box experiments were used to test the social skills of rats.The self-combing experiment was used to test the stereotyped behavior of rats.Hematoxylin eosin(HE)and Nissl staining were used to observe the damage of rat hippocampal tissue and neurons.Enzyme linked immunosor-bent assay(ELISA)was used to measure tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-6 in rat hippocampal tissue.Western blot was used to detect cAMP,CREB,phosphorylated CREB(p-CREB),and BDNF proteins in rat hippocampal tissue.Results:For the control group,the hippocampal tis-sue structure of rats in the model group was damaged,the number of neuronal cells decreased,and the central region residence time,contact time with empty cages,combing frequency,and combing time raised,the TNF-α,IL-1β,IL-6 levels,and the cAMP,p-CREB,and BDNF proteins increased,but the number of up-right times and contact time with unfamiliar mice decreased(P<0.05).For the model group,the hippocam-pal tissue structure and morphology of rats in the baicalin group were greatly improved,the number of neuronal cells raised,and the central region residence time,contact time with empty cages,combing frequency,and combing time reduced,the TNF-α,IL-1β,IL-6 levels,and the cAMP,p-CREB,and BDNF proteins de-creased,but the number of upright times and contact time with unfamiliar mice increased(P<0.05).The cAMP inhibitor SQ22536 can reverse the improvement effect of baicalin on the above indicators in autistic rats(P<0.05).Conclusion:Baicalin may alleviate autism like behavior and neuronal damage in rats with autism by activating cAMP/CREB/BDNF pathway.关键词
自闭症/黄芩苷/环磷酸腺苷/环磷酸腺苷应答元件结合蛋白/脑源性神经营养因子通路/神经元损伤Key words
Autism/Baicalin/Cyclic adenosine monophosphate/cyclic adenosine monophosphate response element binding protein/brain-derived neurotrophic factor pathway/Neuronal damage引用本文复制引用
钟文闻,邹正寿,向庆伟..黄芩苷调控cAMP/CREB/BDNF通路对自闭症大鼠自闭样行为和神经元损伤的影响[J].河北医学,2025,31(11):1787-1793,7.基金项目
湖北省科学技术厅自然科学基金项目,(编号:2022CFD022) （编号:2022CFD022）
