河北医学2025，Vol.31Issue(11)：1815-1821,7.DOI:10.3969/j.issn.1006-6233.2025.11.09
龙胆苦苷调节RhoA/ROCK1信号通路对食管鳞状细胞癌的增殖迁移和侵袭的影响
Effects of Gentiopicroside on the Proliferation Migration and Invasion of Esophageal Squamous Cell Carcinoma by Regulating RhoA/ROCK1 Signaling Pathway
摘要
Abstract
Objective:To explore the effects of gentiopicroside on the proliferation,migration,and inva-sion of esophageal squamous cell carcinoma by regulating Ras homolog family member A(RhoA)/rho A-asso-ciated coiled-coil kinase(ROCK1)signaling pathway.Methods:Human esophageal squamous cell carcino-ma KYSE-180 cells were assigned into untreated group,L-gentiopicroside group,M-gentiopicroside group,H-gentiopicroside group,and H-gentiopicroside+N-arcilasine group.Colony formation assay and CCK-8 were performed to detect cell proliferation.The scratch healing experiment was performed to measure migra-tion.Transwell experiment was performed to measure migration and invasion.Western blot was used to meas-ure the RhoA and ROCK proteins in KYSE-180.Results:Compared with the untreated group,the L-genti-opicroside group,M-gentiopicroside group,and H-gentiopicroside group showed a decrease in OD450 value,scratch healing rate of KYSE-180 cells,the numbers of colony formation,migration,and invasive cells,and the RhoA and ROCK1 proteins(P<0.05),and showed a dose-dependent effect(P<0.05).Compared with the H-gentiopicroside group,the H-gentiopicroside+N-arcilasine group showed an increase in OD450 value,scratch healing rate of KYSE-180 cells,the numbers of colony formation,migration,and invasive cells,and the RhoA and ROCK1 proteins(P<0.05).Conclusion:Gentiopicroside inhibits the RhoA/ROCK1 signaling pathway and suppresses the proliferation,migration,and invasion of esophageal squamous cell carcinoma.关键词
食管鳞状细胞癌/龙胆苦苷/RhoA/ROCK1/增殖/迁移/侵袭Key words
Esophageal squamous cell carcinoma/Gentiopicroside/RhoA/ROCK1/Prolifera-tion/Migration/Invasion引用本文复制引用
言成一,彭建明,顾一敏,袁跃西..龙胆苦苷调节RhoA/ROCK1信号通路对食管鳞状细胞癌的增殖迁移和侵袭的影响[J].河北医学,2025,31(11):1815-1821,7.基金项目
四川省自然科学基金项目,(编号:2025ZNSFSC1864) （编号:2025ZNSFSC1864）
