河北医学2025，Vol.31Issue(11)：1821-1829,9.DOI:10.3969/j.issn.1006-6233.2025.11.010
BbF对慢性肾脏病大鼠肾脏炎症AGE/RAGE信号通路的影响
Effects of Benzo[b]Fluoranthene on Renal Inflammation and AGE/RAGE Signaling Pathway in Rats with Chronic Kidney Disease
摘要
Abstract
Objective:To investigate the effects of benzo[b]fluoranthene(BbF)intervention on renal inflammation and the advanced glycation end products/receptor for advanced glycation end products(AGE/RAGE)signaling pathway in rats with chronic kidney disease(CKD).Methods:A total of 53 specific patho-gen-free(SPF)male Sprague-Dawley(SD)rats were used.Fourteen rats were randomly selected as the control group,and the remaining 39 rats were used to establish a CKD model.Among them,36 rats were suc-cessfully modeled and randomly divided into three groups:the model group(14 rats),the BbF group(11 rats),and the BbF+FPS-ZM1 group(11 rats).The body weight changes of rats in each group were com-pared before and after gavage.After 4 weeks of gavage,a balance was used to measure the weight of both kid-neys and the kidney index;hematoxylin-eosin(HE)staining was used to detect pathological changes in renal tissue.Tail vein blood was collected after 4 weeks of gavage,and a biochemical analyzer was used to detect renal function indicators[serum creatinine(Scr),blood urea nitrogen(BUN),uric acid(UA)].Renal tis-sue was collected after 4 weeks of gavage;enzyme-linked immunosorbent assay(ELISA)was used to detect the content of inflammatory factors[C-reactive protein(CRP),interleukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)]and the levels of oxidative stress[superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)];flow cytometry was used to detect the expres-sion levels of macrophage-related markers[CD11c+,CD206+,CD11c+/CD206+];Western blotting(Wb)was used to detect the levels of the AGE/RAGE signaling pathway.Results:With the extension of gavage time,the body weight of rats in the control group showed a gradual increase trend,while the body weight of rats in the model group,BbF group,and BbF+FPS-ZM1 group all showed a decreasing trend,with a signifi-cant decrease in the BbF group(P<0.05).In addition,the pathological changes in the BbF group were obvi-ous.Compared with the control group,model group,and BbF+FPS-ZM1 group,the BbF group had signifi-cantly increased levels of kidney weight,kidney index,UA,BUN,Scr,IL-6,CRP,TNF-α,IL-8,MDA,CD11c+/CD206+,CD11c+,RAGE mRNA,and AGE mRNA,while the levels of GSH-Px,SOD,and CD206+were significantly decreased,with statistically significant differences(P<0.05).Conclusion:BbF can promote the development of inflammation in CKD rats,accelerate the progression of the disease by activa-ting the AGE/RAGE signaling pathway,and at the same time have adverse effects on oxidative stress,renal function,and macrophages.关键词
苯并[b]荧蒽/慢性肾脏病/肾脏炎症/糖基化终末产物/糖基化终末产物受体信号通路Key words
Benzo[b]fluoranthene/Chronic kidney disease/Renal inflammation/Glycosyla-tion end product/glycosylation end product receptor signaling pathway引用本文复制引用
肖雨歆,王虹英,袁康文,肖胜,杨思洁,邓建军,简讯..BbF对慢性肾脏病大鼠肾脏炎症AGE/RAGE信号通路的影响[J].河北医学,2025,31(11):1821-1829,9.基金项目
四川省区域创新合作项目,(编号:2024YFHZ0081) （编号:2024YFHZ0081）
