河南农业大学学报2025，Vol.59Issue(6)：967-976,10.DOI:10.16445/j.cnki.1000-2340.20250228.001
苦荞FtZF-HD1基因的克隆与表达分析
Cloning and expression analysis of the FtZF-HD1 gene in Fagopyrum tataricum
摘要
Abstract
[Objective]To provide a theoretical basis for further exploring the function of ZF-HD gene in the growth and development of Fagopyrum tataricum,the ZF-HD gene was cloned and its expres-sion pattern was analyzed.[Method]The FtZF-HD1 gene from Tartary buckwheat was cloned using reverse transcription polymerase chain reaction(RT-PCR).Bioinformatics analysis and real-time quantitative PCR(qPCR)were subsequently performed on the gene.[Result]The FtZF-HD1 coding sequence was 606 bp in length and encoded 201 amino acids.There was a homeobox and a zinc finger domain at the C-terminus and N-terminus of FtZF-HD1 protein,respectively.Phylogenetic analysis showed that FtZF-HD1 protein belonged to ZF-HD subfamily protein containing zinc finger domain and was most closely related to ZFHD1 protein in Arabidopsis thaliana.The result of subcellular localiza-tion prediction showed that FtZF-HD1 protein was localized in the nucleus.The q-PCR fluorescence quantitative analysis showed that FtZF-HD1 was expressed in different tissues of Tartary buckwheat,with the highest relative expression in seeds,and its expression pattern in seeds at different develop-mental stages showed a rapid increase followed by a slow decrease and stabilization.[Conclusion]FtZF-HD1 was highly expressed during the middle and late stages of Tartary buckwheat seed develop-ment,suggesting that it play an important regulatory role in the development of Tartary buckwheat seeds.关键词
苦荞/FtZF-HD1基因/基因克隆/生物信息学分析/表达分析Key words
Fagopyrum tataricum/FtZF-HD1 gene/cloning/bioinformatics analysis/expression analysis分类
农业科技引用本文复制引用
冉彬,程远志,张静,刘子洋,孙培媛,李洪有..苦荞FtZF-HD1基因的克隆与表达分析[J].河南农业大学学报,2025,59(6):967-976,10.基金项目
国家自然科学基金项目(31701494,32260461) （31701494,32260461）
国家燕麦荞麦现代农业产业技术体系专项资金(CARS-07-A5) （CARS-07-A5）
贵州省科技计划项目(黔科合基础-ZK[2021]重点035) （黔科合基础-ZK[2021]重点035）
