河南农业大学学报2025，Vol.59Issue(6)：977-987,11.DOI:10.16445/j.cnki.1000-2340.20250116.001
核桃溶血磷脂酸酰基转移酶基因家族分析
Analysis of the gene family of walnut lysophosphatidic acid acyltransferase
摘要
Abstract
[Objective]In order to provide useful information for revealing the molecular mechanisms of oil synthesis and fatty acid accumulation in walnut(Juglans regia),the LPAAT gene family were iden-tified from the walnut whole genome.[Method]The characteristics and functions of LPAAT gene family in walnut were analyzed and predicted by bioinformatics analysis,and the expression patterns of them were also analyzed based on the analysis data of RNA-SEQ(RNA sequencing)in the leaves of walnut seedlings under heat stress.[Result]A total of 13 JrLPAAT members were identified,encoded protein sequences ranged in size from 248 to 384 aa.Most of LPAAT proteins belonged to hydrophobic proteins,and as indicated by subcellular localization analysis,the JrPAAT gene family were primarily located on the endoplasmic reticulum.Phylogenetic analysis divided the JrLPAAT gene family into five subfamilies,with highly similar conserved domains within each subfamily.Transmembrane domains analysis indicated that all members of the JrLPAAT gene family possessed transmembrane structures.The results of cis-acting elements analysis revealed that the JrLPAAT gene family was rich in types of cis-acting elements,including various hormone regulatory,stress-responsive,and light-responsive elements.Intraspecific collinearity analysis revealed five instances of duplication.High-temperature stress expression indicated that,with the exception of JrLPAAT7 and JrLPAAT8,all other genes showed downregulation of varying degrees.[Conclusion]There are 13 JrLPAAT members in the walnut genome,and the structure of members in the same subfamily is conserved.Moreover,JrLPAAT genes are significantly affected by heat stress.关键词
核桃/LPAAT基因家族/油脂合成/生物信息学分析/基因表达Key words
Juglans regia/LPAAT gene family/oil synthesis/bioinformatics analysis/gene expression分类
农业科技引用本文复制引用
杨琳,张党权,赖勇,徐宗乐,崔玮桐,巴丁,样格日,李明婉,陈圆圆,丁申,杨超臣..核桃溶血磷脂酸酰基转移酶基因家族分析[J].河南农业大学学报,2025,59(6):977-987,11.基金项目
国家自然科学基金青年基金项目(32001347) （32001347）
河南省高等学校重点科研项目(22B220003) （22B220003）
河南省博士后科研项目(202103078) （202103078）
