河南农业大学学报2025，Vol.59Issue(6)：1018-1027,10.DOI:10.16445/j.cnki.1000-2340.20250428.002
猪circYME1L1的鉴定及在脂质代谢中的功能预测
Identification of porcine circYME1L1 and functional prediction in lipid metabolism
摘要
Abstract
[Objective]To identify the circular structure of porcine circYME1L1 and to predict its potential functions in lipid metabolism via bioinformatics analyses,thereby providing a theoretical foundation for further investigation into the role of circYME1L1 in lipid metabolism.[Method]Based on previously whole-transcriptome sequencing data from the longissimus dorsi muscle of adult Large White and Queshan Black pigs,circYME1L1 was selected for investigation.Its circular conformation,stability,and subcellular distribution were validated and analyzed via Sanger sequencing,RNase R digestion and subcellular localization assays.Furthermore,miRanda and TargetScan were employed to predict miRNAs interacting with circYME1L1 and their downstream target genes,followed by gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses.[Result]Porcine circYME1L1 is a circular RNA molecule formed through the circularization of exons 9-11 of the YME1L1.It exhibits resistance to RNase R digestion,confirming its circular conformation.Subcel-lular localization analysis revealed its predominant cytoplasmic distribution.Tissue expression profil-ing demonstrated that circYME1L1 is widely expressed across multiple tissues,with significantly higher levels in adipose tissue,lung,and kidney compared to other organs.Bioinformatics analysis identified multiple binding motifs for RNA-binding proteins(AGO,CELF2 and PTBP1)on cir-cYME1L1.A constructed circYME1L1-miRNA-mRNA regulatory network predicted three miRNAs(ssc-miR-31,ssc-miR-149 and ssc-miR-532-3p)and 226 downstream target genes.GO enrichment highlighted terms such as energy metabolism,positive regulation of cell proliferation,growth factor activity,and RNA polymerase Ⅱ regulation.KEGG pathway analysis revealed significant enrichment in lipid metabolism-related pathways,including PI3K-Akt signaling,MAPK signaling,fat digestion/absorption,and adipocytokine signaling.Based on the above results and bioinformatics analysis,cir-cYME1L1 regulated gene expression associated with lipid metabolism as a competitive endogenous RNA(ceRNA).[Conclusion]Porcine circYME1L1 stably exists in vivo and may play a regulatory role in lipid metabolism,offering novel insights for further mechanistic exploration.关键词
猪/亚细胞定位/circYME1L1/靶基因预测/脂质代谢Key words
pigs/subcellular localization/circYME1L1/prediction target gene/lipid metabolism分类
农业科技引用本文复制引用
常乐彬,王克君,柏峻,韩雪蕾,王冰洁,王畅,刘贤,李新建,王腾飞,王春秀,李秀领,余彤..猪circYME1L1的鉴定及在脂质代谢中的功能预测[J].河南农业大学学报,2025,59(6):1018-1027,10.基金项目
科技创新2030-重大项目(2023ZD04046) （2023ZD04046）
"十四五"国家重点研发计划项目(2021YFD1301202) （2021YFD1301202）
河南省农业良种联合攻关项目(2022020101) （2022020101）
