环境工程学报2025，Vol.19Issue(10)：2414-2425,12.DOI:10.12030/j.cjee.202411129
中国南方某大型自来水厂中自由生活阿米巴的分布特征研究
Study on the distribution characteristics of free-living amoebae in a large urban water supply plant in southern China
摘要
Abstract
Free-living amoebae(FLA)are of crucial importance due to their role as both potential pathogens to human health and key ecological players in aquatic environments.This study employed a combination of culture-based and molecular techniques to investigate the distribution patterns of FLA and their associated intracellular bacteria in a large water treatment plant located in the lower Yangtze River Basin.A total of 107 samples were collected from various stages of the treatment plant during three sampling events.The total positive rate of FLA was 39.3％.FLA were mainly distributed in the raw water and the initial treatment stages,but none FLA were detected in the effluent of the plant,indicating that the pre-ozonation,horizontal sedimentation,sand filtration,activated carbon filtration,and disinfection were effective in removing FLA originating from raw water and other units,with filtration units being the primary FLA removal stages.A total of 42 FLA strains were isolated and purified,and 28 operational taxonomic units(OTUs)were identified after 18s rRNA gene sequencing,which belong to three genera:Naegleria,Vermamoeba and Acanthamoeba.Among them,Naegleria was the dominant amoeba genus in the plant,followed by Acanthamoeba and Vermamoeba.And the distribution of Acanthamoeba and Vermamoeba in the plant may be influenced by temperature and dissolved oxygen concentration.Several potential human pathogenic bacteria,such as Pseudomonas species,Bacillus species,Paenibacillus species,Escherichia-Shigella,and Aeromonas species,as well as environmentally significant bacteria involved in carbon and nitrogen metabolism,such as Comamonas,Brevibacillus,Pelomonas,Aquabacterium,and Herminiimonas,were detected within the cells of isolated FLA.Among them,Pseudomonas,Brevibacillus and Bacillus were the core intracellular bacteria in FLA of the plant.The detection of FLA-harbored pathogenic bacteria(e.g.,Pseudomonas,Bacillus)in water treatment units necessitates plant upgrades to eliminate chlorine-resistant pathogens.The results of this study can provide technical and data support for improving the safety of water supply in the Yangtze River Basin.关键词
自来水厂/自由生活阿米巴/分离培养/胞内菌Key words
water supply plant/free-living amoebae/cultivation/amoeba-intracellular bacteria分类
资源环境引用本文复制引用
董倩芮,田哲,韩子铭,段福明,张辉灿,张昱,杨敏..中国南方某大型自来水厂中自由生活阿米巴的分布特征研究[J].环境工程学报,2025,19(10):2414-2425,12.基金项目
国家重点研发计划资助项目(2022YFC3204700) （2022YFC3204700）
天津市科技计划资助项目(22YFXTHZ00080) （22YFXTHZ00080）
