河南农业科学2025，Vol.54Issue(12)：62-69,8.DOI:10.15933/j.cnki.1004-3268.2025.12.007
金线莲类原球茎生物反应器培养体系的建立
Establishment of a Bioreactor Culture System for Anoectochilus roxburghii Protocorm-Like Bodies
摘要
Abstract
To screen the optimal hormone combination and germplasm for inducing Anoectochilus roxburghii protocorm-like bodies(PLBs),and to investigate the effects of inoculum density and culture duration on PLBs biomass and kinsenoside content for optimizing large-scale culture conditions,this study utilized tissue-cultured seedlings of Anoectochilus roxburghii.The effects of different concentration combinations of 6-benzylaminopurine(6-BA),1-naphthaleneacetic acid(NAA),zeatin(ZT),and 2,4-dichlorophenoxyacetic acid(2,4-D)on the induction of PLBs from five different Anoectochilus roxburghii germplasms were tested.The PLB induction rate,kinsenoside content,and proliferation characteristics were compared among the different plant germplasms.Using a plant bioreactor,the influence of inoculum density(20-80 g/L)and culture duration(10-40 days)on biomass and kinsenoside synthesis was studied.The results indicated that the combination of MS+3 mg/L 6-BA+0.5 mg/L NAA+0.8 mg/L ZT+0.2 mg/L 2,4-D medium had the best effect on improving the induction rate of adventitious buds of different species of Anoectochilus roxburghii germplasms.Under identical culture conditions,the ZY1(Nanjing,Fujian)germplasm demonstrated superior PLB induction rate(93%),kinsenoside content(5.03%),and radial proliferation capacity compared to other germplasms.In the bioreactor culture system,both the proliferation coefficient(1.46)and kinsenoside content(7.80%)of the PLBs peaked at an inoculum density of 60 g/L after 40 days of culture in darkness.In conclusion,utilizing the superior ZY1 germplasm with the MS+3 mg/L 6-BA+0.5 mg/L NAA+0.8 mg/L ZT+0.2 mg/L 2,4-D medium,combined with bioreactor culture conditions of 60 g/L inoculum density and 40 days of culture in darkness,enables the highly efficient accumulation of both Anoectochilus roxburghii PLB biomass and kinsenoside content.关键词
金线莲/类原球茎/生物反应器/金线莲苷Key words
Anoectochilus roxburghii/Protocorm-like body/Bioreactor/Kinsenoside分类
农业科技引用本文复制引用
章方玲,孔向军,周小军,王红珍,吴梅,方莉..金线莲类原球茎生物反应器培养体系的建立[J].河南农业科学,2025,54(12):62-69,8.基金项目
浙江省科技重大项目(农业新品种选育专项子课题)(2021C02074-4-3) （农业新品种选育专项子课题）
金华市科技局农业重大科研项目(2022-2-009,2023-2-2005) （2022-2-009,2023-2-2005）
