化学工程2025，Vol.53Issue(11)：26-31,6.DOI:10.3969/j.issn.1005-9954.2025.11.005
一株瘤胃嗜热厌氧产纤维乙醇菌的定向分离与发酵
Isolation and fermentation of thermophilic anaerobic cellulosic ethanol-producing strain from rumen
摘要
Abstract
The natural microbial community domestication strategy within the context of CBP(consolidated bioprocessing technology)was utilized to establish a targeted strain selection system,aiming to isolate thermophilic anaerobic spore-forming bacteria capable of lignocellulose degradation and cellulosic ethanol production.Via morphological examination,physiological and biochemical profiling,and 16S rDNA sequencing,strain XK1-1 was identified as Caldibacillus thermoamylovoran.The results show that under anaerobic conditions at 55 ℃ and 180 r/min,employing a glucose and xylose(mass volume ratio 2∶1 g/L)mixed carbon source during continuous cultivation for 12 h,strain XK1-1 realizes a maximal ethanol yield of(1.03±0.046)g/L,demonstrating heightened metabolic responsiveness to glucose.Furthermore,within a medium exclusively utilizing Salix as the carbon source and undergoing continuous fermentation for 48 h,the cellulose degradation rate by the strain uphold at 35％±0.21％,the hemicellulose degradation rate is at 53％±0.85％,and the ethanol yield is(0.27±0.05)g/L(significance p＞0.05).Hence,this investigation furnishes high-quality strains and theoretical underpinnings for the advancement of non-food biomass fuels.关键词
生物燃料/纤维乙醇/木质纤维素/瘤胃/微生物菌群/嗜热厌氧菌Key words
biofuels/cellulosic ethanol/lignocellulose/rumen/microbial community/thermophilic anaerobes分类
生物科学引用本文复制引用
徐凯,崔金娜,魏国光,刘占英..一株瘤胃嗜热厌氧产纤维乙醇菌的定向分离与发酵[J].化学工程,2025,53(11):26-31,6.基金项目
国家自然科学基金资助项目(22568032) （22568032）
内蒙古自治区重点产业链创新联合体专项(2025ZDLH0006) （2025ZDLH0006）
