四川大学学报（医学版）2025，Vol.56Issue(5)：1208-1214,7.DOI:10.12182/20250960103
梯度渐变量子点荧光技术在布鲁氏菌属快速检测中的应用评价
Evaluation of Gradient-Variant Quantum Dot Fluorescence Technology for Rapid Detection of Brucella
摘要
Abstract
Objective To establish a brucellosis monitoring and testing technique applicable for the rapid field screening of natural epidemic diseases.Methods A rapid testing technique for Brucella was developed based on a double-antibody sandwich testing model using gradient-variant quantum dots as fluorescent tracers.The sensitivity,linearity,precision,and specificity of the technique were evaluated using suspensions of standard Brucella strains.Methodological comparisons across different sample types were conducted to assess the consistency of the test results.Results The gradient-variant quantum dots detection method was evaluated with standard Brucella strains,exhibiting a sensitivity of 1×103 CFU/mL and a linear correlation coefficient(r)of 0.994(95%CI,0.933-1.055).The maximum coefficient of variation was 12.94%in repeated tests,showing good specificity.A comparative assessment of 305 clinical samples was conducted using the Brucella gradient-variant quantum dots detection method,the Rose Bengal plate agglutination test(RBT),and the serum agglutination test(SAT),yielding a Kappa value of 0.95,indicating almost perfect agreement.Additionally,a comparative assessment of 110 environmental samples collected on-site was conducted using the Brucella gradient-variant quantum dots detection method and quantitative real-time PCR(qPCR).The Kappa values for aerosol collection fluid,surface wipes,and wool samples were all above 0.83,demonstrating near-perfect agreement.For fecal and soil samples,the Kappa values were above 0.62,indicating substantial agreement.Conclusion The Brucella detection method based on gradient-variant quantum dots technology is simple and can be conducted rapidly.The detection method demonstrates high sensitivity,linearity,precision,and specificity.It shows consistent performance in clinical sample testing.It is well-suited for field rapid screening of natural epidemic diseases in field settings and shows good application prospects in the monitoring,prevention,and rapid detection of zoonotic diseases.关键词
量子点/布鲁氏菌/快速检测/亲和层析Key words
Quantum dots/Brucella/Rapid test/Affinity chromatography引用本文复制引用
李积权,周蕾,王昕桐,王建玲,田胜男,赵存哲,王晓晨,简文秀,李生瑜,张青雯..梯度渐变量子点荧光技术在布鲁氏菌属快速检测中的应用评价[J].四川大学学报（医学版）,2025,56(5):1208-1214,7.基金项目
科技创新基地建设项目(No.2024ZY021)资助 （No.2024ZY021）
