杭州师范大学学报（自然科学版）2025，Vol.24Issue(6)：625-632,8.DOI:10.19926/j.cnki.issn.1674-232X.2024.10.291
白豆杉实时荧光定量PCR内参基因筛选
Screening of Reference Genes of Pseudotaxus chienii by Real-time Fluorescent Quantitative PCR
摘要
Abstract
Pseudotaxus chienii,a monotypic genus within the Taxaceae family endemic to China,is a rare and protected species.To identify suitable reference genes for gene expression analysis in different tissues of P.chienii,this study selected six candidate reference genes(B1-B6)based on existing transcriptome data.Their expression stability was evaluated across various tissues(root,stem,old leaf,and new leaf)using real-time fluorescent quantitative PCR(RT-qPCR)and analyzed with five methods:geNorm,NormFinder,BestKeeper,comparative △Ct method,and RefFinder.The results indicated that B3 was the most stably expressed gene across all tested tissues,whereas B2 was the least stable.Thus,B3 is recommended as the optimal reference gene for future functional gene expression studies in P.chienii.关键词
白豆杉/内参基因/实时荧光定量PCRKey words
Pseudotaxus chienii/reference gene/real-time fluorescent quantitative PCR分类
生物科学引用本文复制引用
马若云,方子瑾,张玲晓,郑梦颖,白恩惠,沈晨佳..白豆杉实时荧光定量PCR内参基因筛选[J].杭州师范大学学报（自然科学版）,2025,24(6):625-632,8.基金项目
国家自然科学基金项目(32271905) （32271905）
浙江省自然科学基金项目(LY23C160001). （LY23C160001）
