家畜生态学报2025，Vol.46Issue(11)：15-20,6.DOI:10.3969/j.issn.1673-1182.2025.11.003
miR-21对奶山羊乳腺上皮细胞增殖和乳成分调控作用的研究
Effect of miRNA-21 on the Proliferation and Regulation of Milk Composition of Dairy Goat Mammary Epithelial Cells
摘要
Abstract
To investigate the regulatory effects of miR-21 on the proliferation and milk component of goat mammary epithelial cells(GMECs),miR-21 mimics and inhibitors were transfected into GMECs.The expression levels of apoptosis-and prolifer ation-relates genes Bcl-21 and Bax,as well as the secretion of β-casein and triglgerides(TG),were examined using EdU,CCK-8,ELISA,RT-qPCR,and Western Blot.Results indicated that the viability of GMECs in the miR-21 inhibitor group was significantly reduced(P＜0.01),while apoptosis levels were significantly increased(P＜0.01).In contrast,the viability of GMECs was significantly increased in the miR-21 mimics group(P＜0.05).There was a significant in-crease in the secretion of β-casein and TG in GMECs transfected with miR-21 mimics(P＜0.01),whereas interference with miR-21 inhibitors significantly decreased the secretion of β-casein and TG in GMECs(P＜0.01).Compared with the control group,phosphorylation of PI3K,mTOR,S6K,and ERK proteins significantly increased in the miR-21 mimics group(P＜0.01).Bax protein expression was significantly suppressed(P＜0.01),while Bcl-2 protein expression was significantly upregulated(P＜0.01).In the miR-21 inhibitors group,the phosphorylation of PI3K,mTOR,S6K,and ERK proteins significantly de-creased(P＜0.05),Bax protein expression was significantly upregulated(P＜0.01),and Bcl-2 protein ex-pression was significantly downregulated(P＜0.05).The results indicate that miR-21 suppresses apoptosis in goat mammary epithelial cells by activating the ERK/Bcl-2/Bax signaling pathway and promotes the se-cretion of β-casein and TG through activation of the PI3K/mTOR/S6K signaling pathway.关键词
乳腺上皮细胞/miR-21/奶山羊/凋亡/β-酪蛋白/TGKey words
mammary epithelial cells/miR-21/diary goat/apoptosis/β-casein/TG分类
农业科技引用本文复制引用
段屈雨,吕兰兰,程泽芳,严天荣,赵金男,张玉洲,赵涛涛,马毅,安小鹏..miR-21对奶山羊乳腺上皮细胞增殖和乳成分调控作用的研究[J].家畜生态学报,2025,46(11):15-20,6.基金项目
陕西省重点研发计划(2021NY-001,2020ZDLNY02-01) （2021NY-001,2020ZDLNY02-01）
合水县教育和科学技术局(QY-HS-2023A-01) （QY-HS-2023A-01）
