基础医学与临床2025，Vol.45Issue(12)：1541-1547,7.DOI:10.16352/j.issn.1001-6325.2025.12.1541
急性和慢性肾脏病小鼠模型中肾组织SFXNs表达下降
Decreased expression of SFXNs in renal tissues of mouse models of acute and chronic kidney disease
摘要
Abstract
Objective To investigate the expression changes of iron autophagy-mitochondrial ferric ion transport protein families(SFXNs)in acute kidney injury(AKI)and chronic kidney disease(CKD)mouse models induced by cisplatin(Cis)and ischemia reperfusion(IR).Methods C57BL/6 mice were randomly divided into control group(control),Cis-AKI group,Cis-CKD group,sham-operated group(sham),IR-AKI group,and IR-CKD group.Serum and kidney tissue samples were collected from mice.Serum creatinine(Cr)and blood urea nitrogen(BUN)levels were detected.Pathological changes in renal tissues were observed by HE staining.Western blot was used to detect the expression of renal SFXNs and kidney injury related proteins.Results Compared with the control or sham group,the levels of BUN and Cr in the serum of the model group were significantly increased(P＜0.05),the renal tissue showed significant pathological damage,with the kidney injury molecule-1(KIM-1),neutrophil gelatinase-associated lipocalin(NGAL),and pro-apoptotic protein Bax significantly upregulated(P＜0.05),while the anti-apoptotic protein Bcl-2 was significantly downregulated(P＜0.05).Compared to the control or sham group,the Cis-AKI group showed a significant downregulation of SFXN4(P＜0.05);The SFXN4 and SFXN5 subtypes were significantly downregulated in the IR-AKI group and Cis-CKD group(P＜0.05);All five subtypes of SFXN in the IR-CKD group were significantly downregulated(P＜0.05).Conclusions Cis or IR in-duces renal tissue damage and tubular mitochondrial injury in mice and affects the expression of SFXN family pro-teins,suggesting their potential role in renal injury of animal models.关键词
顺铂/缺血再灌注/急性肾损伤/慢性肾脏病/铁自噬-线粒体铁离子转运蛋白家族Key words
cisplatin/ischemia reperfusion/acute kidney injury/chronic kidney disease/sideroflexins分类
医药卫生引用本文复制引用
高立,王思懿,张敏菁,赵琳,徐哲明,张根生,严洁萍..急性和慢性肾脏病小鼠模型中肾组织SFXNs表达下降[J].基础医学与临床,2025,45(12):1541-1547,7.基金项目
浙江省自然科学基金联合基金(LHDMY23H070007) （LHDMY23H070007）
浙江省医药卫生科技计划项目(2025KY011) （2025KY011）
