基础医学与临床2025，Vol.45Issue(12)：1557-1564,8.DOI:10.16352/j.issn.1001-6325.2025.12.1557
骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤
Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury
摘要
Abstract
Objective To explore the effects and mechanisms of bone marrow mesenchymal stem cell(BMMSC)-derived exosomes(BMMSC-Exo)on hypoxia/reoxygenation(H/R)-induced injury in rat cardiomyocyte cell line(H9c2).Methods BMMSC-Exosomes were isolated by ultracentrifugation.The cells were divided into three groups:control,H/R,and H/R+BMMSC-Exo(H/R+Exo).A hypoxia/reoxygenation(H/R)injury model was es-tablished by exposing cells to 12 hours of hypoxia followed by 6 hours of reoxygenation.Flow cytometry was used to detect cell apoptosis,DHE staining was used to assess cellular ROS levels,JC-1 immunofluorescence staining was used to evaluate mitochondrial membrane potential,and Western blot was used to detect mitochondrial autophagy-re-lated proteins.Results BMMSC-Exo treatment significantly alleviated oxidative stress,restored mitochondrial mem-brane potential,reduced mitochondrial autophagy levels,and effectively decreased cardiomyocyte apoptosis.Conclu-sions Bone marrow mesenchymal stem cell-derived exosomes alleviate H/R-induced cardiomyocyte injury.关键词
外泌体/低氧/复氧/氧化应激/线粒体/凋亡Key words
exosomes/hypoxia/reoxygenation/oxidative stress/mitochondria/apoptosis分类
医药卫生引用本文复制引用
文雯,刘晨曦,陈双景,陆晓炯,靳志涛,张铮..骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤[J].基础医学与临床,2025,45(12):1557-1564,8.基金项目
北京市自然科学基金(7222229) （7222229）
