基础医学与临床2025，Vol.45Issue(12)：1565-1571,7.DOI:10.16352/j.issn.1001-6325.2025.12.1565
miR-132-3p通过靶向调控SIRT1/NF-κB通路影响滋养层细胞上皮-间质转化
Effect of miR-132-3p on epithelial-mesenchymal transition of trophoblast cells by targeting the SIRT1/NF-κB pathway
摘要
Abstract
Objective To investigate the effect of miR-132-3p on epithelial-mesenchymal transition(EMT)of trophoblast cells through targeted regulation of the SIRT1/NF-κB pathway.Methods Placental tissues from 37 pre-eclampsia(PE)patients who underwent cesarean section in Zhengzhou Women&Infants Hospital and 37 normal pregnant women who underwent cesarean section were collected as the research group and control group,respectively.RT-qPCR was performed to detect the expression of miR-132-3p and SIRT1 in tissues.During the logarithmic pro-liferation phase,HTR-8/SVneo cells were grouped into inhibitor negative group,miR-132-3p inhibitor group,mimic negative group,miR-132-3p overexpression group,miR-132-3p inhibitor+interference negative group,and miR-132-3p inhibitor+interference SIRT1 group,with untransfected HTR-8/SVneo cells as the blank group.CCK-8,Transwell assay,scratch assay,and RT-qPCR were used to detect the proliferation,invasion,migration,and changes in miR-132-3p and SIRT1 mRNA expression of HTR-8/SVneo cells.Western blot was used to detect the expression levels of EMT related proteins and SIRT1,NF-κB proteins.Dual luciferase was used to validate the targeting relationship between miR-132-3p and SIRT1.Results The level of miR-132-3p in the research group was significantly higher than that in the control group,while the SIRT1 mRNA was lower(P＜0.05).The level of miR-132-3p,E-cadherin,p-NF-κBp65/NF-κBp65 in the miR-132-3p inhibitor group was lower than those in the inhib-itor negative group and blank group.The proliferation rate,invasion cell number,migration rate,level of vimentin,N-cadherin,and SIRT1 were all higher(P＜0.05).The miR-132-3p,E-cadherin,p-NF-κBp65/NF-κBp65 in the miR-132-3p overexpression group were higher than those in the mimic negative group,the proliferation rate,inva-sion number,migration rate,vimentin,N-cadherin,and SIRT1 were lower(P＜0.05).The level of E-cadherin and p-NF-κBp65/NF-κBp65 in the miR-132-3p inhibitor+interference SIRT1 group was higher than those in the miR-132-3p inhibitor+interference negative group(P＜0.05),the proliferation rate,invasion number,migration rate,vimentin,N-cadherin,and SIRT1 expression were lower(P＜0.05).MiR-132-3p targeted regulation of SIRT1 expression(P＜0.05).Conclusions Inhibition of miR-132-3p promotes the EMT,proliferation,invasion,and migration of trophoblast cells by targeting and activating the SIRT1/NF-κB pathway.关键词
上皮-间质转化/SIRT1/NF-κB通路/miR-132-3p/滋养层细胞Key words
epithelial-mesenchymal transition/SIRT1/NF-κB pathway/miR-132-3p/trophoblast cells分类
医药卫生引用本文复制引用
郭书焕,高亚丽,陈静鸽..miR-132-3p通过靶向调控SIRT1/NF-κB通路影响滋养层细胞上皮-间质转化[J].基础医学与临床,2025,45(12):1565-1571,7.基金项目
河南省医学科技攻关计划联合共建项目(LHGJ20220873) （LHGJ20220873）
