基础医学与临床2025，Vol.45Issue(12)：1572-1579,8.DOI:10.16352/j.issn.1001-6325.2025.12.1572
miR-423-5p在缺血性卒中患者血浆中的表达升高
Elevated expression of miR-423-5p in the plasma of patients with ischemic stroke
摘要
Abstract
Objective To explore the clinical significance of miR-423-5p in ischemic stroke and its effects on SH-SY5Y cell apoptosis along with the underlying mechanisms.Methods Plasma samples were collected from three stroke-prone patients who subsequently developed acute ischemic stroke(AIS)within 24 hours.High-throughput sequencing was used to identify miR-423-5p as a key target.Venous blood samples were collected from 46 AIS pa-tients within 24 hours of onset and from 46 matched control cases.RT-qPCR was performed to measure the plasma expression of miR-423-5p in both groups.The diagnostic value of miR-423-5p for AIS was evaluated using receiver operating characteristic(ROC)curve.After overexpression and knockdown of miR-423-5p in SH-SY5Y cells,flow cytometry was performed to detect apoptosis in each group to analyze the potential relationship between miR-423-5p and SH-SY5Y cell apoptosis.Western blot was used to measure changes in the expression level of the ap-optotic protein cleaved caspase-3 and the anti-apoptotic proteins Bcl-2 and Bcl-xL.Results The expression of miR-423-5p in 3 AIS patients showed significant difference before and after onset.Plasma miR-423-5p expression was significantly elevated in AIS patients versus controls(P＜0.001).ROC analysis indicated its strong diagnostic potential for AIS.Overexpression of miR-423-5p increased the apoptosis rate and cleaved caspase-3 ex-pression of SH-SY5Y cells after OGD/R,and decreased the expression of Bcl-2 and Bcl-xL,while knocking down miR-423-5p had the opposite effect.Conclusions The expression of miR-423-5p is upregulated in the plasma of patients with ischemic stroke compared to healthy controls.Furthermore,miR-423-5p promotes OGD/R-induced apoptosis in SH-SY5Y cells by increasing the level of cleaved caspase-3 and suppressing the expression of Bcl-2 and Bcl-xL.关键词
miR-423-5p/缺血性卒中/生物标志物/细胞凋亡Key words
miR-423-5p/ischemic stroke/biomarker/apoptosis分类
医药卫生引用本文复制引用
李海鹏,刘一帆,李珊,陈道辉,雷京霖,胡政..miR-423-5p在缺血性卒中患者血浆中的表达升高[J].基础医学与临床,2025,45(12):1572-1579,8.基金项目
湖南省卫生健康委国家临床重点专科重大科研专项(Z2023127) （Z2023127）
湖南省自然科学基金(2023JJ50372) （2023JJ50372）
湖南省卫生健康委科研计划项目(D202303077684) （D202303077684）
湘南学院校级科研项目(2022JX109) （2022JX109）
