基础医学与临床2025，Vol.45Issue(12)：1580-1587,8.DOI:10.16352/j.issn.1001-6325.2025.12.1580
miR-141-3p下调溶血磷脂酸受体3抑制脑胶质瘤细胞增殖、迁移和上皮-间质转化
miR-141-3p down-regulating lysophosphatidic acid receptor3 inhibits proliferation,migration and epithelial-mesenchymal transition of brain glioma cells
摘要
Abstract
Objective To investigate the impacts of miR-141-3p on the proliferation,migration and epithelial-mesen-chymal transformation of glioma cells by regulating lysophosphatidic acid receptor 3(LPAR3).Methods RT-qPCR was used to detect the level of miR-141-3p and LPAR3 in glioma tissues and cells.Dual luciferase was used to de-tect the targeting relationship between miR-141-3p and LPAR3.The cells were divided into control group,miR-NC group,miR-141-3p mimics group,miR-141-3p mimics+pcDNA3.1 group,and miR-141-3p mimics+pcDNA-LPAR3 group,and then transfected with corresponding plasmids.RT-qPCR was used to detect the level of miR-141-3p and LPAR3 in cells.EdU method was used to detect cell proliferation.The scratch healing experiment was used to detect cell migration.Western blot was used to detect the expression of proteins related to cell proliferation,migration,and epithelial-mesenchymal transformation.Xenograft tumor model in nude mice was used to observe tumor formation.RT-qPCR was used to detect the level of miR-141-3p in tumor tissue.In addition,Western blot was performed to detect the expression of LPAR3,PCNA,and MMP-2.Results miR-141-3p was downregulated,whereas LPAR3 mRNA was upregulated in glioma tissues and U251,T98G,and CHG-5 cell lines(P＜0.05).There was a targeted binding site between miR-141-3p and LPAR3.miR-141-3p mimics significantly increased the ex-pression of miR-141-3p and E-cadherin,but decreased LPAR3 mRNA level,EdU-positive rate,scratch wound healing rate,and the expression of PCNA,cyclin D1,MMP-2,MMP-9,N-cadherin,and vimentin(P＜0.05).pcDNA-LPAR3 reversed effect on expression of these factors(P＜0.05).Tumor transplantation experiments in nude mice showed that miR-141-3p mimics reduced tumor volume,tumor weight,LPAR3,PCNA,and MMP-2 expres-sion,and increased the level of miR-141-3p(P＜0.05).Conclusions miR-141-3p can inhibit proliferation,mi-gration,and epithelial-mesenchymal transformation of glioma cells by down-regulating LPAR3.关键词
miR-141-3p/溶血磷脂酸受体 3/脑胶质瘤细胞/增殖/上皮-间质转化Key words
miR-141-3p/lysophosphatidic acid receptor 3/glioma cell/proliferation/epithelial-mesenchymal transformation分类
医药卫生引用本文复制引用
李文辉,任红岗,郭剑,宋洋,冯富强..miR-141-3p下调溶血磷脂酸受体3抑制脑胶质瘤细胞增殖、迁移和上皮-间质转化[J].基础医学与临床,2025,45(12):1580-1587,8.基金项目
山西省基础研究计划(20210302123265) （20210302123265）
山西省卫生健康委科研课题(2021060) （2021060）
