基础医学与临床2025，Vol.45Issue(12)：1593-1599,7.DOI:10.16352/j.issn.1001-6325.2025.12.1593
敲低PIAS3缓解血糖波动诱导的大鼠心肌细胞系H9c2氧化应激及线粒体功能障碍
Knockdown of PIAS3 alleviates glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in rat cardiomyocyte cell line H9c2
摘要
Abstract
Objective To investigate the effect of PIAS3 on glucose fluctuation-induced oxidative stress and mito-chondrial dysfunction in rat cardiomyocytes.Methods H9c2 were cultured in vitro,and divided into normal glucose control group(Control),mannitol-induced osmotic pressure control group(MG),constant high glucose group(HG),intermittent hyperglycemia group(IHG),IHG+siRNA NC group,and IHG+PIAS3 siRNA group.Cell proliferation was assessed using CCK-8 assay.LDH release,MDA and GSH levels,as well as SOD activity,were detected using corresponding kits.Mitochondrial membrane potential was evaluated via JC-1 staining combined with flow cytometry.ROS levels in cells and mitochondria were determined using DCFH-DA and MitoSOX staining,re-spectively.Protein expression of PI3K,p-PI3K,AKT,and p-AKT was analyzed by Western blot.Results Com-pared with the control group,intermittent hyperglycemia promoted oxidative stress and mitochondrial dysfunction,significantly upregulated PIAS3 expression(P＜0.001)and downregulated p-PI3K and p-AKT protein levels(P＜0.001).Knockdown of PIAS3 significantly alleviated oxidative stress and mitochondrial dysfunction induced by glucose fluctuations,and increased p-PI3K and p-AKT protein levels(P＜0.001).Conclusions Knockdown of PIAS3 may alleviate glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in ratcardiomyocytes by activating the PI3K/AKT signaling pathway.关键词
PIAS3/血糖波动/氧化应激/线粒体功能障碍Key words
PIAS3/glucose fluctuation/oxidative stress/mitochondrial dysfunction分类
医药卫生引用本文复制引用
成永霞,于龙,李化敏,赵硕,张怡扬,刘贵波..敲低PIAS3缓解血糖波动诱导的大鼠心肌细胞系H9c2氧化应激及线粒体功能障碍[J].基础医学与临床,2025,45(12):1593-1599,7.基金项目
黑龙江省医药卫生科研课题(20220101040735) （20220101040735）
黑龙江省省属高等学校基本科研业务费攀登项目(2022-KYYW-FMY-0002) （2022-KYYW-FMY-0002）
