江苏大学学报（医学版）2025，Vol.35Issue(6)：461-467,7.DOI:10.13312/j.issn.1671-7783.y250073
血浆胞外囊泡LncRNA TUG1在非小细胞肺癌中的表达及意义
Expression and clinical values of LncRNA TUG1 of plasma extracellular vesicles in non-small cell lung cancer
摘要
Abstract
Objective:To screen taurine upregulated gene 1(TUG1),a long non-coding RNA(LncRNA)with highly specific differential expression,in plasma extracellular vesicles(EVs)from patients with non-small cell lung cancer(NSCLC),and to validate its clinical value as a diagnostic biomarker for NSCLC.Methods:LncRNA sequencing was performed on plasma EVs from 6 NSCLC patients and 6 healthy individuals.Quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression of LncRNA TUG1 in plasma EVs from 60 NSCLC patients,60 healthy controls,and 93 patients for differential diagnosis(including small cell lung cancer,mediastinal lymphoma,etc.).The correlation between TUG1 expression and clinical characteristics of NSCLC was analyzed.The effect of TUG1 on the biological behavior of NSCLC cells was explored through in vitro cellular experiments,and the receiver operating characteristic(ROC)curve was used to evaluate its diagnostic efficacy.Results:Sequencing and qRT-PCR verification showed that TUG1 expression in plasma EVs of NSCLC patients was significantly higher than that in healthy individuals(P＜0.01),and its expression in multiple NSCLC cell lines was higher than that in normal bronchial epithelial cells.Notably,TUG1 expression in plasma EVs of NSCLC patients decreased significantly after surgery(P＜0.01).TUG1 expression was correlated with tumor size,distant metastasis,lymph node metastasis,and TNM stage(P＜0.05 or P＜0.01).Knockdown of TUG1 significantly inhibited the proliferation and migration abilities of H1299 cells(P＜0.05 or P＜0.01).ROC curve analysis showed that the area under the curve(AUC)of TUG1 for diagnosing NSCLC was 0.849(specificity=0.83,sensitivity=0.86).When combined with carcino-embryonic antigen(CEA),the AUC was further improved to 0.882(P＜0.01).Conclusion:LncRNA TUG1 is highly expressed in plasma EVs of NSCLC patients and closely associated with tumor progression.It can serve as a novel diagnostic biomarker for NSCLC,and combined detection with CEA can enhance diagnostic efficacy.关键词
LncRNA TUG1/非小细胞肺癌/血浆/胞外囊泡/诊断标志物Key words
LncRNA TUG1/non-small cell lung cancer(NSCLC)/plasma/extracellular vesicles/diagnostic biomarker分类
医药卫生引用本文复制引用
夏雪,戴锦东,宁芳婕,沈健,彭辉勇,朱栋炜..血浆胞外囊泡LncRNA TUG1在非小细胞肺癌中的表达及意义[J].江苏大学学报（医学版）,2025,35(6):461-467,7.基金项目
国家自然科学基金青年基金资助项目(82202001) （82202001）
张家港市科技计划项目(ZKYL2456) （ZKYL2456）
苏州市科技计划项目(SLT2022001) （SLT2022001）
