江苏大学学报（医学版）2025，Vol.35Issue(6)：480-486,549,8.DOI:10.13312/j.issn.1671-7783.y250045
冷冻消融术后坏死液对非小细胞肺癌A549和H1299细胞增殖和凋亡的影响及其机制
The effects and mechanisms of cryoablation-derived necrotic fluid on the proliferation and apoptosis of non-small cell lung cancer A549 and H1299 cells
摘要
Abstract
Objective:To investigate the effects of cryoablation-derived necrotic fluid on the proliferation and apoptosis of non-small cell lung cancer(NSCLC)A549 and H1299 cells,and to analyze its immune-related mechanisms.Methods:NSCLC A549 and H1299 cell lines were selected and divided into control groups(conventional culture)and necrotic fluid groups(supplemented with cryoablation-induced necrotic fluid).Cell proliferation was assessed by using CCK-8 assay,and apoptosis was detected by flow cytometry.Indirect co-culture systems were established by using human peripheral blood mononuclear cells(PBMCs)and two cell lines.The human tumor microenvironment research chip(AAH-CYT-G5)was employed to detect 80 immune-related proteins,followed by differential protein screening,clustering and GO/KEGG pathway enrichment analysis.Results:Compared with the control group,the cell proliferation rates of the A549 and H1299 cells in the necrosis fluid group were significantly decreased(P＜0.05),and the cell apoptosis rates were markedly increased(P＜0.05).The results of the co-culture experiment showed that compared with the control group,68 differentially expressed proteins of the two type cells were detected in A549 cells in the necrosis fluid group(P＜0.05),with KEGG enrichment analysis identifying 76 pathways;for H1299 cells,70 differentially expressed proteins were detected(P＜0.05),with KEGG enrichment analysis identifying 74 pathways.The top 10 common differentially expressed proteins of the two type cells were GM-CSF,IP-10,NAP-2,SDF-1,FGF-6,IL-6,Osteopontin and fractalkine.Core pathways were identified as PI3K-Akt signaling,MAPK signaling,TNF signaling,JAK-STAT signaling,IL-17 signaling and Ras signaling.Conclusion:The lysate released by necrotic tumor cells after cryoablation could inhibit the proliferation of NSCLC A549 and H1299 cells and promote their apoptosis.It may exert anti-tumor effect by modulating immune-related proteins and key signaling pathways to influence the tumor immune microenvironment.关键词
非小细胞肺癌/冷冻消融/肿瘤免疫微环境/坏死液/人肿瘤微环境研究芯片Key words
non-small cell lung cancer/cryoablation/tumor immune microenvironment/necrotic fluid/human tumor microenvironment research chip分类
医药卫生引用本文复制引用
刘一凡,梁天宇,王玥慧,刘殿娜,张语馨,李泉旺..冷冻消融术后坏死液对非小细胞肺癌A549和H1299细胞增殖和凋亡的影响及其机制[J].江苏大学学报（医学版）,2025,35(6):480-486,549,8.基金项目
国家自然科学基金面上项目(82174458) （82174458）
国家自然科学基金青年科学基金项目(82405515) （82405515）
国家重点研发计划资助项目(2018YFC1705102) （2018YFC1705102）
中央高校基本科研业务费专项资金资助北京中医药大学重点攻关项目(2020-JYB-ZDGG-127) （2020-JYB-ZDGG-127）
