江苏大学学报（医学版）2025，Vol.35Issue(6)：496-503,8.DOI:10.13312/j.issn.1671-7783.y240117
含SET结构域赖氨酸甲基转移酶5对卵巢癌细胞增殖和迁移的影响
Effect of SET domain containing lysine methyltransferase 5 on proliferation and migration of ovarian cancer cells
摘要
Abstract
Objective:To investigate the expression and significance of SET domain containing lysine methyltransferase 5(SETD5)in ovrian cancer tissues and cell lines.Methods:The correlation between SETD5 mRNA expression and the prognosis of patients with ovarian cancer was analyzed by Kaplan-Meier survival curves based on public database.Immunohistochemical staining was used to detect the expression level of SETD5 in 88 cases of ovarian cancer tissues and 8 cases of normal ovarian tissues.Western blotting was used to detect the expression levels of SETD5 in ovarian granulosa cells KGN,ovarian endometrioid adenocarcinoma cells A2780,ovarian serous adenocarcinoma cells SKOV3,epithelial ovarian cancer cells Hey A8 and ovarian serous adenocarcinoma cells ES-2.The SKOV3 cell line of ovarian serous adenocarcinoma was selected to construct a stable overexpression cell line of SETD5,and two SETD5-targeting shRNAs were used to construct a stable knockdown cell line of ovarian endometrioid adenocarcinoma A2780 cell line.The migration ability of SKOV3 and A2780 cells was detected by Transwell and wound healing assays,and the proliferation ability of SKOV3 and A2780 cells was detected by cell counting and CCK-8 assay.Results:The Kaplan-Meier survival analysis curves showed that high expression of SETD5 mRNA was associated with shortened progression-free survival and overall survival in patients with ovarian cancer.The immunohistochemical results of tissue microarray showed that the positive expression rate of SETD5 protein in ovarian cancer tissues was significantly higher than that in normal ovarian tissues(x2=7.308,P=0.007).The expression level of SETD5 was significantly correlated with the tumor diameter(P=0.003),T stage(P=0.017),and FIGO stage(P=0.012)of patients with ovarian cancer.The results of in vitro functional experiments showed that overexpression of SETD5 significantly promoted the proliferation and migration ability of SKOV3 cells,while knockdown of SETD5 significantly inhibited the proliferation and migration of A2780 cells.Conclusion:SETD5 is highly expressed in human ovarian cancer tissues as well as SKOV3 and A2780 cells,and is associated with the prognosis of patients,promotes the proliferation and migration of ovarian cancer cells.关键词
SETD5/预后/迁移/增殖/卵巢癌Key words
SET domain containing 5/prognosis/migration/proliferation/ovarian cancer分类
医药卫生引用本文复制引用
侯雅楠,张紫薇,张博文,黄婷,林琼,邵根宝..含SET结构域赖氨酸甲基转移酶5对卵巢癌细胞增殖和迁移的影响[J].江苏大学学报（医学版）,2025,35(6):496-503,8.基金项目
江苏省重点研发计划项目(BE2020678) （BE2020678）
