菌物研究2025，Vol.23Issue(5)：399-407,9.DOI:10.13341/j.jfr.2024.1700
糙皮侧耳热激转录因子鉴定及其基因表达模式分析
Identification and Analysis of Gene Expression Patterns of Heat Shock Transcription Factors in Pleurotus ostreatus
摘要
Abstract
To explore functions of heat shock transcription factors responsable to environmental stress and growth development of macrofungi,five proteins carrying the DNA binding domain of Hsf family were identified from Pleurotus ostreatus,as named as PoHsf1-PoHsf5,respectively.Bioinformatics analysis showed that all of the five PoHsfs belonged to unstable and hydrophilic proteins and were located in the cell nucleus.Detection of conserved motifs and phylogenetic analysis using the full protein sequences indicated that PoHsf1 and PoHsf4 were highly related to ScHsf1 while PoHsf2 was closely similar to ScSkn7 of Saccharomyces cerevisiae.Analysis of gene expression patters demonstrated that the activity of PoHsf1 was significantly downregulated during the two stages of primordial formation and fruiting body as well as under the high temperature stress but was significantly upregulated under the low temperature and hydrogen peroxide stress.The expression activity of both PoHsf2 and PoHsf3 was decreased during the primordial formation stage.Significant upregulation of exression activity was observed for PoHsf4 under the high temperature stress and for PoHsf5 during the primordial formation and fruiting body stages,respectively.The above study thus should provide a useful foundation for future understanding of the functional roles of heat shock transcription factors particularly on the stress responses in P.ostreatus.关键词
食用菌/糙皮侧耳/热激转录因子/生物信息学/表达模式Key words
edible fungi/Pleurotus ostreatus/heat shock transcription factor/bioinformatics/expression pattern分类
农业科技引用本文复制引用
于振,麦非凡,刘元栋,胡延如,文晴,戚元成,王风芹..糙皮侧耳热激转录因子鉴定及其基因表达模式分析[J].菌物研究,2025,23(5):399-407,9.基金项目
国家现代农业产业技术体系专项资金项目(CARS-20),河南省农业良种联合攻关项目(2022030101),河南省重大公益专项(201300110700) （CARS-20）
