辽宁农业科学Issue(6)：15-21,7.DOI:10.3969/j.issn.1002-1728.2025.06.003
养殖用水中40种常用兽药同时筛查检测方法的建立
Establishment of Simultaneous Screening and Detection Methods for 40 Commonly Used Veterinary Drugs in Water
摘要
Abstract
s:This article establishes a method for simultaneous screening and detection of 40 commonly used veterinary drug residues in aquaculture water using ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The influence of different pretreatment conditions and instrument conditions on the detection results was examined to select the optimal solution.The target substance was enriched and puri-fied using an HLB solid-phase extraction column,separated using a Shim-pack GIST C18 chromatographic col-umn,and confirmed by mobile UPLC-MS/MS with acetonitrile and a 5 mmol/L ammonium acetate solution containing 0.1％formic acid.Quantification was performed using an external standard method.The quantita-tive limit of this method is 2～10 ng/L,recovery rate 83％～108％,relative standard deviation 3.8％～10.3％.Compared with traditional detection methods,it has the advantages of detecting a wide variety of com-pounds,low detection limit,good stability,simplicity and speed.At the same time,this method was applied to detect and analyze samples from aquaculture farms in Liaoning Province,to determine the residues and me-tabolism of 40 veterinary drugs in 9 categories of aquatic product aquaculture water.This provides technical support for better controlling the safety and quality of aquatic product food in the market,the aquaculture envi-ronment,and the discharge and reuse of aquaculture wastewater.关键词
兽药残留/养殖用水/快速分散固相萃取/液相色谱—串联质谱Key words
Veterinary drug residues/Aquaculture water/QuEChERS/UPLC-MS/MS分类
农业科技引用本文复制引用
张彤,张敏,付海滨,吴渺渺,刘宝山,徐蕾蕊,韩小虎,王素华,康明芹,林天驰..养殖用水中40种常用兽药同时筛查检测方法的建立[J].辽宁农业科学,2025,(6):15-21,7.基金项目
海关总署科研计划项目(2024HK028) （2024HK028）
辽宁省自然科学基金项目(2023-MS-213) （2023-MS-213）
