现代畜牧兽医Issue(11)：18-24,7.DOI:10.20154/j.cnki.issn1672-9692.2025.11.004
基于网络药理学和分子对接技术的黄芩抑制杀鲑气单胞菌的作用机制分析
Analysis of the mechanism of action of Scutellaria baicalensis in inhibiting Aeromonas salmonicida based on network pharmacology and molecular docking technology
摘要
Abstract
This research employed network pharmacology and molecular docking techniques to investigate the inhibitory mechanism of Scutellaria baicalensis against Aeromonas salmonicida.The susceptibility of Aeromonas salmonicida JMAV14 to the drug was screened through in vitro antibacterial assays of traditional Chinese medicine,and the minimum inhibitory concentration(MIC)was determined.Active components of Scutellaria baicalensis were collected from the TCMSP database,and their targets were obtained via the PubChem,Swiss Target Prediction,and Swiss ADME databases.Targets related to'Aeromonas salmonicida'were retrieved from the Genecards,OMIM,and TTD databases.Intersecting targets were identified using the Microbioinformatics Venny platform.Protein-protein interaction(PPI)analysis of the intersecting targets was performed on the STRING platform,while GO functional enrichment and KEGG pathway enrichment analyses were conducted on the DAVID platform.Molecular docking was carried out using AutoDockTools software.The results showed that 36 active components and 445 potential targets were screened from Scutellaria baicalensis,with 13 targets associated with inhibiting Aeromonas salmonicida,yielding two intersecting targets.PPI analysis revealed that Bcl2l1 and Mcl1 were core targets.GO and KEGG enrichment analyses identified 16 functional terms and three pathways related to the inhibition of Aeromonas salmonicida,with the apoptosis pathway being the most significantly affected.Both wogonin and acacetin were able to bind to the core targets MCL1 and BCL2L1.The study demonstrates that active components in Scutellaria baicalensis,such as wogonin and acacetin,act on the targets Bcl2l1 and Mcl1,inhibiting Aeromonas salmonicida by regulating the apoptosis pathway,JAK-STAT signaling pathway,and PI3K-Akt signaling pathway.关键词
黄芩/杀鲑气单胞菌/网络药理学/分子对接/抑菌机制Key words
Scutellaria baicalensis/Aeromonas salmonicida/Network pharmacology/Molecular docking/Mechanism分类
农业科技引用本文复制引用
王谦,孙弋雅,梁学超,崔苗苗,薛红波,董筱萍,刘丽颖,刘英姿..基于网络药理学和分子对接技术的黄芩抑制杀鲑气单胞菌的作用机制分析[J].现代畜牧兽医,2025,(11):18-24,7.基金项目
辽宁省教育厅基本科研面上项目(LJKMZ20221254) （LJKMZ20221254）
辽宁省科技厅面上项目(2024-MS-194) （2024-MS-194）
辽宁省营口英才计划项目(XLYC2213009) （XLYC2213009）
辽宁省科技特派项目(2025JH5/10400052) （2025JH5/10400052）
