内蒙古民族大学学报（自然科学版）2025，Vol.40Issue(6)：1-8,8.DOI:10.14045/j.cnki.15-1220.2025.06.001
适配体SELEX技术及其在生物医学中的应用
Aptamer SELEX Technology and Its Applications in Biomedicine
摘要
Abstract
Aptamers are artificially synthesized oligonucleotide fragments screened from oligonucleotide libraries through systematic evolution of ligands by exponential enrichment(SELEX)technology,and they possess the char-acteristics of high affinity and high specificity binding to targets.During the screening process,one of the key steps is to isolate the oligonucleotide fragments with strong binding ability to the target from the library,with the aim of obtaining a high-quality functional aptamer sequence.Since its inception,the SELEX technology has undergone continuous in-novation and improvement,covering a wide range of new technologies based on various targets and platforms such as cells,capillary electrophoresis,magnetic beads,graphene oxide,and microfluidic chips,and has been successfully applied to aptamers screening.This paper reviews the concept of aptamers,the differences between aptamers and antibodies,the diverse methods of aptamer screening,and their practical applications in the biomedical field,with the aim of providing a reference basis for the subsequent screening of aptamers and the application research in related fields.关键词
适配体/SELEX/生物医学Key words
aptamer/SELEX/biomedicine分类
医药卫生引用本文复制引用
翟景波,杨元超,肖人方..适配体SELEX技术及其在生物医学中的应用[J].内蒙古民族大学学报（自然科学版）,2025,40(6):1-8,8.基金项目
内蒙古自治区重点研发和成果转化计划项目(2025YFSH0054) （2025YFSH0054）
内蒙古自治区直属高校基本科研业务费项目(NMDJK24002) （NMDJK24002）
内蒙古自治区2025年财政项目(251402) （251402）
内蒙古自治区研究生教育教学改革项目(JG2024045C) （JG2024045C）
新时代"科尔沁英才"工程创新团队项目(KEQYC20240202) （KEQYC20240202）
