内蒙古民族大学学报（自然科学版）2025，Vol.40Issue(6)：23-33,11.DOI:10.14045/j.cnki.15-1220.2025.06.004
荞麦NADP依赖型苹果酸酶基因家族分析
Analysis of NADP-malic Enzyme Gene Family in Buckwheat
摘要
Abstract
NADP-dependent malic enzyme(NADP-ME)is a pivotal metabolic enzyme that plays critical roles in regulating plant photosynthesis and stress responses.Buckwheat(Fagopyrum),known for its unique nutritional components,comprises two commonly cultivated species:common buckwheat(Fagopyrum esculentum)and tartary buckwheat(Fagopyrum tataricum).In this study,five members of the NADP-ME gene family were identified from the whole genomes of common and tartary buckwheat,respectively,and designated as FeNADP-ME1,FeNADP-ME2,FeNADP-ME3,FeNADP-ME4,FeNADP-ME5 and FtNADP-ME1,FtNADP-ME2,FtNADP-ME3,FtNADP-ME4,FtNADP-ME5.The encoded proteins of buckwheat NADP-ME range from 568 to 649 amino acid residues,with molecular weights varying between 62.89 and 70.88 kDa.All buckwheat NADP-ME proteins are acidic and hydrophobic,lack transmembrane structures,and are primarily localized in chloroplasts,cytoplasm,and plasma membranes.Analysis of the protein's structure reveals that α-helices and random coils constitute the major com-ponents of the secondary structure of the buckwheat NADP-ME family proteins.Multiple sequence alignment re-sults indicate that these family members contain five highly conserved structural regions(Region Ⅰ～Region Ⅴ).Phylogenetic analysis classifies buckwheat NADP-ME into subgroups Ⅱ,Ⅲ and Ⅴ within the phylogenetic tree.Further investigation demonstrates that the conserved motifs of buckwheat NADP-ME are intact.Specifically,FeNADP-ME1～FeNADP-ME5 and FtNADP-ME1～FtNADP-ME5 encompass Motif 1～Motif 12 and Motif 15,whereas FeNADP-ME4,FeNADP-ME5 and FtNADP-ME4,FtNADP-ME5 contain all 15 conserved motifs and pos-sess both the Malic-M domain and a typical split gene structure.Members of the buckwheat NADP-ME family are distributed across four respective chromosomes,with FeNADP-ME1,FeNADP-ME2 and FtNADP-ME1,FtNADP-ME2 showing collinearity with the NADP-ME genes of Arabidopsis thaliana.Analysis of the cis-acting elements of buckwheat NADP-ME members uncovers multiple light-responsive,plant hormone-responsive,and stress-responsive elements.The analysis of cis-acting elements in buckwheat NADP-ME members identified multiple regu-latory elements responsive to light,plant hormones,and stress.Among these,light-responsive elements were the most abundant(56.52%),followed by hormone-responsive elements such as methyl jasmonate(MeJA)and cold stress-responsive elements(LTR).关键词
NADP依赖型苹果酸酶/荞麦/基因家族/生物信息学分析Key words
NADP-malic enzyme/buckwheat/gene family/bioinformatics analysis分类
农业科技引用本文复制引用
王欣颖,齐景凯,霍红雁,吕静波,柳研娣,靳亚楠..荞麦NADP依赖型苹果酸酶基因家族分析[J].内蒙古民族大学学报（自然科学版）,2025,40(6):23-33,11.基金项目
内蒙古自治区自然科学基金项目(2025MS03068) （2025MS03068）
内蒙古自治区科技计划项目(2022YFDZ0043) （2022YFDZ0043）
内蒙古自治区直属高校基本科研业务费项目(GXKY25Z002) （GXKY25Z002）
内蒙古自治区第七批"草原英才"工程创新团队——高产、高生物黄酮荞麦新品种选育及标准化栽培技术研究创新人才团队项目(2022) （2022）
