北方农业学报2025，Vol.53Issue(4)：49-66,18.DOI:10.12190/j.issn.2096-1197.2025.04.05
基于转录组学的沙芥耐盐基因挖掘
Mining of salt-tolerance genes in Pugionium cornutum based on transcriptomics
摘要
Abstract
[Objective]To screen key genes in the transcriptome of stress-tolerant germplasm resources of Pugionium cornutum under salt stress,thereby laying a foundation for elucidating the physiological research of salt-tolerance and stress resistance in P.cornutum,and providing a reference for molecular breeding of salt-tolerance in P.cornutum.[Methods]The leaf fresh weight ratio of P.cornutum seedlings at the 6-leaf-1-heart stage was measured after 8 days of treatment with 0,50,100,150,and 200 mmol/L NaCl.The salt concentration corresponding to approximately 50%of the fresh weight ratio was chosen as the treatment concentration for transcriptome analysis,with sampling conducted at 0,3,and 12 h for transcriptome sequencing.[Results]The salt threshold of P.cornutum was determined to be 100 mmol/L by measuring the leaf fresh weight ratio;compared with the 0 h,transcriptomic data at 3 and 12 h identified 10 006 and 14 117 differentially expressed genes,respectively;a total of four upregulated genes were screened among the transcription factors.In the plant hormone signal transduction process,brassinosteroid(BR)had the highest number of upregulated genes;three key upregulated enzymes were identified in the phenylpropanoid metabolic pathway,and four key upregulated enzymes were identified in the flavonoid biosynthesis pathway.[Conclusion]The upregulated genes involved in transcription factors,the plant hormone signal transduction process,the phenylpropanoid metabolic pathway,and the flavonoid biosynthesis pathway may play critical roles in the response of P.cornutum to salt stress.关键词
沙芥/转录组/盐胁迫/耐盐基因Key words
Pugionium cornutum/Transcriptome/Salt stress/Salt-tolerance gene分类
农业科技引用本文复制引用
商凯凡,赵昀暐,廉勇,王萍..基于转录组学的沙芥耐盐基因挖掘[J].北方农业学报,2025,53(4):49-66,18.基金项目
国家自然科学基金项目(31860553,31260475) （31860553,31260475）
