青岛大学学报（医学版）2025，Vol.61Issue(5)：649-653,5.DOI:10.11712/jms.2096-5532.2025.61.154
丰富环境对缺血性脑卒中小鼠运动和认知功能影响
Effect of enriched environment on the motor and cognitive functions of mice with ischemic stroke
摘要
Abstract
Objective To investigate the effect of enriched environment(EE)on the motor and cognitive functions of mice with middle cerebral artery occlusion/reperfusion(MCAO/R)and its underlying mechanism.Methods A mouse model of MCAO/R was constructed,and then 1 day later,the mice were fed in EE or standard environment for 14 days.The modified Neu-rological Severity Score(mNSS)and the rotarod test were used to evaluate the motor function of mice;the Y-maze test was used to evaluate the cognitive function of mice;Western blotting was used to measure the expression level of vesicular glutamate trans-porter 1(VGLUT1)in the cortex,striatum,and hippocampus of the ipsilateral side.Results EE significantly reduced the mNSS score of MCAO/R mice(F=103.900,q=9.551,P＜0.001)and increased fall latency in the rotarod test,the percentage of time spent in the novel arm,and the percentage of entries into the novel arm in MCAO/R mice(F=10.110-31.910,q=4.271-7.769,P＜0.05),and it also reduced the expression level of VGLUT1 in the cortex,striatum,and hippocampus of the ipsilateral hemisphere of MCAO/R mice(F=8.141-14.600,q=5.318-7.240,P＜0.05).Conclusion EE can significantly improve the motor and cognitive disorders in mice with MCAO/R,possibly by downregulating the expression of VGLUT1.关键词
环境疗法/卒中/缺氧缺血,脑/动作障碍/认知障碍/囊泡谷氨酸转运蛋白质1/小鼠Key words
milieu therapy/stroke/hypoxia-ischemia,brain/movement disorders/cognition disorders/vesicular glutamate transport protein 1/mice分类
医药卫生引用本文复制引用
董闪闪,朱婷,孟萍萍,王玉阳,王强..丰富环境对缺血性脑卒中小鼠运动和认知功能影响[J].青岛大学学报（医学版）,2025,61(5):649-653,5.基金项目
山东省自然科学基金项目(ZR2021QH143,ZR20-20MH282) （ZR2021QH143,ZR20-20MH282）
