青岛大学学报（医学版）2025，Vol.61Issue(5)：654-658,5.DOI:10.11712/jms.2096-5532.2025.61.149
黄芩苷对血管性痴呆大鼠认知功能的影响及其机制
Effects of baicalin on cognitive function in rats with vascular dementia and associated mechanism
摘要
Abstract
Objective To investigate the effects of baicalin on cognitive function in a rat model of vascular dementia(VD)and its mechanism of action.Methods A rat VD model was prepared by permanent ligation of bilateral common carotid arteries.VD rats were randomly divided into VD model group(VD),low-dose baicalin group(Bcn-L),high-dose baicalin group(Bcn-H),and high-dose baicalin plus ML385 group(Bcn-H+ML385).A sham-operation group(sham)was set up.Twelve rats were as-signed to each group.The Bcn-L,Bcn-H,and Bcn-H+ML385 groups were given baicalin(intragastrically at 25 mg/kg),baicalin(intragastrically at 100 mg/kg),and baicalin(intragastrically at 100 mg/kg)followed by ML385(intraperitoneally at 30 mg/kg),respectively,while the sham and VD groups were given the same volume of the solvent,for continuous 28 days.The learning and memory abilities of rats were assessed through the Morris water maze test.Histopathological changes in the hippocampus were examined with HE staining.The levels of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH),and fer-rous ion(Fe2+)in the hippocampus of rats were determined by biochemical assays.The protein expression levels of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase 1(HO-1),glutathione peroxidase 4(GPX4),and ferritin heavy chain 1(FTH1)in the hippocampus of rats were measured by Western blot.Results Compared with the sham group,the VD group showed notable lesions in the hippocampal tissue;a significantly longer escape latency and significantly fewer platform crossings;significantly higher MDA and Fe2+levels in the hippocampal tissue;and significantly lower levels of GSH,SOD,Nrf2,HO-1,GPX4,and FTH1 in the hippocampus(F=151.906-781.377,all P＜0.05).Compared with the VD group,the Bcn-H group showed alleviated hippocampal lesions;a significantly shorter escape latency and significantly more platform crossings;significantly lower MDA and Fe2+levels in the hippocampus;and significantly higher GSH,SOD,Nrf2,HO-1,GPX4,and FTH1 levels in the hippocampus(all P＜0.05).There were no significant differences in the above indices between the Bcn-L group and the VD group(all P＞0.05).Compared with the Bcn-H group,the Bcn-H+ML385 group showed worsened hippocampal lesions;a significantly longer escape latency and significantly fewer platform crossings;significantly higher MDA and Fe2+levels in the hippocampus;and significantly lower GSH,SOD,Nrf2,HO-1,GPX4,and FTH1 levels in the hippocampus(all P＜0.05).Conclusion Baicalin may improve cognitive function in VD rats by up-regulating the Nrf2/GPX4 pathway,blocking ferroptosis,and reducing hippocampal tissue damage.关键词
黄芩苷/痴呆,血管性/铁死亡/氧化性应激/大鼠Key words
baicalin/dementia,vascular/ferroptosis/oxidative stress/rats分类
医药卫生引用本文复制引用
董俊杰,张玲,王英,马丽..黄芩苷对血管性痴呆大鼠认知功能的影响及其机制[J].青岛大学学报（医学版）,2025,61(5):654-658,5.基金项目
新疆维吾尔自治区自然科学基金(2020D0-1C111) （2020D0-1C111）
