青岛大学学报（医学版）2025，Vol.61Issue(5)：659-664,6.DOI:10.11712/jms.2096-5532.2025.61.156
CHK2调节PARP1对血管性痴呆大鼠神经功能影响
Effect of checkpoint kinase 2 on neurological function in rats with vascular dementia by regulating poly(ADP-ribose)polymerase 1
摘要
Abstract
Objective To investigate the effect of checkpoint kinase 2(CHK2)on neurological function in rats with vascular dementia(VD)and its mechanism by regulating poly(ADP-ribose)polymerase 1(PARP1).Methods A total of 46 adult male Sprague-Dawley rats were divided into negative control group with 13 rats,model group with 13 rats,CHK2 shRNA group with 10 rats,and PARP1 shRNA group with 10 rats.HE staining was used to observe the pathological changes of hippocampal tissue and neurons in rats;TUNEL assay was used to detect the apoptosis of hippocampal neurons in rats;the Morris water maze test was used to evaluate the changes in the number of platform crossings and escape latency;ELISA was used to measure the expres-sion of interleukin-1β,interleukin-6,and tumor necrosis factor-α in the hippocampal tissue of rats;colorimetry was used to mea-sure the expression of superoxide dismutase(SOD),reduced glutathione(GSH),and malondialdehyde(MDA)in hippocampal tis-sue;Western blotting was used to measure the expression of CHK2 and PARP1 in hippocampal tissue.Results There were sig-nificant differences in the mean values of each indicator between multiple groups(F=46.850-607.692,P＜0.001).Compared with the model group,the CHK2 shRNA group and the PARP1 shRNA group had improvement in the damaged hippocampal tis-sue structure,a significant reduction in the number of apoptotic hippocampal neurons(P＜0.05),a significant increase in the num-ber of platform crossings,and a significant reduction in escape latency(P＜0.05),as well as significant reductions in the expres-sion of inflammatory factors and MDA and significant increases in the expression of SOD and GSH in hippocampal tissue(P＜0.05).The CHK2 shRNA group had a significant reduction in the expression of PARP1 in hippocampal tissue(P＜0.05).Con-clusion Inhibiting the expression of CHK2 in VD rats can improve their cognitive function,possibly by interfering with CHK2 to reduce the expression of PARP1.关键词
痴呆,血管性/周期检测点激酶2/多(ADP核糖)聚合酶1/炎症介导素类/氧化性应激/记忆与学习测试/大鼠,Sprague-DawleyKey words
dementia,vascular/checkpoint kinase 2/poly(ADP-ribose)polymerase-1/inflammation mediators/oxidative stress/memory and learning tests/rats,Sprague-Dawley分类
医药卫生引用本文复制引用
姚婷,邓谦,周颖,石红梅,蒋军林,吴玉华..CHK2调节PARP1对血管性痴呆大鼠神经功能影响[J].青岛大学学报（医学版）,2025,61(5):659-664,6.基金项目
湖南省自然科学基金青年基金项目(2022JJ40242) （2022JJ40242）
