陕西中医2025，Vol.46Issue(12)：1587-1592,6.DOI:10.3969/j.issn.1000-7369.2025.12.001
电针通过cAMP-PKA/ATF2轴促进白色脂肪米色化产热预防肥胖机制研究
Study on the mechanism of electroacupuncture in preventing obesity by promoting white fat beige thermogenesis through cAMP-PKA/ATF2 axis
摘要
Abstract
Objective:To investigate whether electroacupuncture(EA)therapy promotes beige thermogenesis of white fat through the cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)/activating transcription fac-tor 2(ATF2)signaling axis,thereby preventing the occurrence of obesity.Methods:40 C57 BL/6J mice were random-ly divided into 4 groups,10 in each group:Control(Ctrl)group,Model group,EA group and EA+H-89 group.The body weight,Lee's index and blood lipid index of mice in each group were detected.HE staining was employed to ob-serve the morphological alterations in abdominal adipose tissue.The protein expression levels of PR domain contai-ning 16(PRDM16),uncoupling protein 1(UCP1),peroxisome proliferator-activated receptor gamma coactivator 1 alpha(PGC-1α),PKA and ATF2 were detected by Western blotting.The body weight,Lee's index and blood lip-cAMP was detected by ELISA.Results:Compared with the Ctrl group,the body weight,Lee's index,total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)content and white fat mass of the Model group were significantly increased.High-density lipoprotein cholesterol(HDL-C)content,PRDM16,UCP1,PGC-1α,PKA and ATF2 protein expression levels,and cAMP content were significantly reduced(P<0.05).Compared with the Model group,the body weight,Lee's index,TC,TG,LDL-C content and white fat mass of the EA group were signifi-cantly decreased.The expression levels of PRDM16,UCP1,PGC-1 lipid index of mice in each cAMP content were significantly increased(P<0.05).Compared with the EA group,the body weight,Lee's index,TC,TG,LDL-C con-tent and white fat mass of the EA+H-89 group were significantly increased.The protein expression levels of PRDM16,UCP1,PGC-1roup were detected.cAMP content were significantly decreased(P<0.05).Conclusion:EA can activate the cAMP-PKA/ATF2 signaling pathway,and then up-regulate the expression levels of key regulatory factors PRDM16,UCP1 and PGC-1αin the process of white adipose tissue beige,promote the transformation of white fat to beige fat,enhance the body's heat production efficiency,and play a role in weight loss and lipid reduction.关键词
肥胖/电针/白色脂肪米色化/环磷酸腺苷/蛋白激酶 A/激活转录因子 2Key words
Obesity/Electroacupuncture/White fat beige/Cyclic adenosine monophosphate/Protein kinase A/Activating transcription factor 2分类
医药卫生引用本文复制引用
付珞,田劲,冯超,刘雁军..电针通过cAMP-PKA/ATF2轴促进白色脂肪米色化产热预防肥胖机制研究[J].陕西中医,2025,46(12):1587-1592,6.基金项目
国家自然科学基金资助项目(82170887) （82170887）
