陕西中医2025，Vol.46Issue(12)：1593-1597,1615,6.DOI:10.3969/j.issn.1000-7369.2025.12.002
玉屏风散对变应性鼻炎大鼠肥大细胞表达、免疫应答及NLRP3炎性小体的影响
Effects of Yupingfeng powder on the expression and immune response of NLRP3 inflammasome in allergic rhinitis rats
摘要
Abstract
Objective:To investigate the effects of Yupingfeng powder on mast cell expression,immune response and NLRP3 inflammasome in rats with allergic rhinitis.Methods:Forty rats were randomly allocated to four groups(n=10 per group):normal,model,mometasone furoate,and Yupingfeng powder.Allergic rhinitis was induced in the model,mometasone furoate,and Yupingfeng powder groups via ovalbumin sensitization,while the normal group re-mained untreated.Following successful model establishment,the mometasone furoate group received one spray per nostril of mometasone furoate nasal spray daily.The Yupingfeng powder group was administered 24 g/kg of Yuping-feng powder via oral gavage.Concurrently,the normal and model groups received equivalent volumes of saline.Aller-gic symptom scores were recorded for all animals.Histopathological evaluation of nasal mucosa and mast cell detec-tion were performed using HE staining and toluidine blue staining,respectively.Serum levels of immune-related fac-tors were measured by ELISA,and Western blot analysis was used to determine the expression of NLRP3 inflamma-some-associated proteins in nasal mucosal tissues.Results:Compared to the normal group,the model group exhibited significant increases in allergic symptom scores,mast cell count in nasal mucosa,serum levels of OVA-sIgE and IL-4,as well as protein expression of NLRP3,Caspase-1,and ASC in nasal mucosa tissue(P<0.05),along with a marked reduction in serum INF-γlevel(P<0.05).Relative to the model group,both the mometasone furoate and Yupingfeng powder groups showed reductions in allergic symptom scores,nasal mucosal mast cell numbers,OVA-sIgE and IL-4 concentrations,and NLRP3 inflammasome-related protein expressions(P<0.05),while demonstra-ting a significant elevation in serum INF-γ(P<0.05).Furthermore,the improvements in the Yupingfeng powder group were significantly more pronounced than those in the mometasone furoate group(P<0.05).Histopathological evaluation also indicated substantial amelioration of nasal mucosal lesions in both treatment groups compared to the model group.Conclusion:Yupingfeng powder can effectively regulate the immune response,reduce mast cell expres-sion and inhibit NLRP3 inflammasome in rats with allergic rhinitis.关键词
变应性鼻炎/玉屏风散/NLRP3 炎性小体/大鼠/肥大细胞/免疫应答Key words
Allergic rhinitis/Yupingfeng powder/NLRP3 inflammasome/Rats/Mast cell/Immune response分类
医药卫生引用本文复制引用
施晓卉,顾蒙泰,沈花,周凌..玉屏风散对变应性鼻炎大鼠肥大细胞表达、免疫应答及NLRP3炎性小体的影响[J].陕西中医,2025,46(12):1593-1597,1615,6.基金项目
国家自然科学基金资助项目(82204929) （82204929）
江苏省苏州市科技发展计划项目(SYSD2020196) （SYSD2020196）
