陕西中医2025，Vol.46Issue(12)：1598-1603,6.DOI:10.3969/j.issn.1000-7369.2025.12.003
针刺对血虚风燥型特应性皮炎豚鼠模型表皮通透屏障功能的调控机制研究
Study on the regulatory mechanism of acupuncture on epidermal permeability barrier function in a guinea pig model of atopic dermatitis with blood deficiency and wind-dryness pattern
摘要
Abstract
Objective:To investigate the regulatory mechanism of acupuncture on epidermal permeability barrier function in a guinea pig model of atopic dermatitis(AD)with blood deficiency and wind-dryness pattern.Methods:32 ordinary-grade pure white male guinea pigs were randomly divided into a model group,blank group,control group,and acupuncture group.Except for the blank group,the other groups were used to establish an AD model with blood deficiency and wind-dryness pattern by topical application of 2,4-dinitrochlorobenzene(DNCB)acetone solution.The model group,blank group,and acupuncture group were given distilled water via gavage.Based on this,the acupunc-ture group received acupuncture at bilateral Feishu(BL 13),Pishu(BL 20),Geshu(BL 17),and Quchi(LI 11).The control group was administered loratadine tablets via gavage.All interventions lasted for 14 days.Skin lesion severity was evaluated[Eczema Area and Severity Index(EASI)score],barrier function was assessed[stratum corneum wa-ter content(WCSC),transepidermal water loss(TEWL)],immune markers(IgE,IL-4,IL-17)were measured by ELISA,AQP3 expression was detected by Western blot,and AQP3 mRNA expression in lesional skin tissue was measured by reverse transcription polymerase chain reaction(RT-PCR).Results:Compared with the blank group,the model group showed significantly increased EASI scores,TEWL,and levels of IgE,IL-4,and IL-17(P<0.05),and significantly decreased WCSC,AQP3 expression,and AQP3 mRNA expression in lesional skin tissue(P<0.05).Compared with the model group,the acupuncture group showed significantly decreased EASI scores,TEWL,and lev-els of IgE,IL-4,and IL-17(P<0.05),and significantly increased WCSC,AQP3 expression,and AQP3 mRNA ex-pression in lesional skin tissue(P<0.05).Conclusion:Acupuncture intervention in the guinea pig model of AD with blood deficiency and wind-dryness pattern effectively ameliorated epidermal water regulation and epidermal permea-bility barrier function.Its mechanism of action is related to regulating the immune stress response and the expression levels of AQP3 and AQP3 mRNA in lesional skin tissue.关键词
特应性皮炎/针刺/血虚风燥型/表皮通透屏障/水通道蛋白 3/调控机制Key words
Atopic dermatitis/Acupuncture/Blood deficiency and wind-dryness pattern/Epidermal permeabil-ity barrier/Aquaporin 3/Mechanisms of regulation分类
医药卫生引用本文复制引用
吴乐,汪秀梅,周光..针刺对血虚风燥型特应性皮炎豚鼠模型表皮通透屏障功能的调控机制研究[J].陕西中医,2025,46(12):1598-1603,6.基金项目
新疆维吾尔自治区自然科学基金资助项目(2022D01C781) （2022D01C781）
