陕西中医2025，Vol.46Issue(12)：1604-1609,6.DOI:10.3969/j.issn.1000-7369.2025.12.004
肾衰泄浊汤对慢性肾衰大鼠肾脏巨噬细胞向肌成纤维细胞转换的影响
Effect of Shenshuai Xiezhuo decoction on the transition of renal macrophages to myofibroblasts in rats with chronic renal failure
摘要
Abstract
Objective:To evaluate the effect of Shenshuai Xiezhuo decoction on macrophage-to-myofibroblast transition(MMT)in the kidneys of rats with chronic renal failure(CRF)induced by oral administration of adenine,and to explore the potential underlying mechanism via the miR-142-5p/SOCS1/JAK3 axis.Methods:Twenty-four Sprague-Dawley rats were randomly divided into four groups(n=6 per group):normal control group,model group,low-dose traditional Chinese medicine(TCM)group,and high-dose TCM group.Except for the normal group,CRF was induced in all other groups by oral administration of adenine for 21 days.After modeling,rats in the normal and model groups were administered normal saline,while those in the low-dose and high-dose TCM groups received Shenshuai Xiezhuo decoction at the corresponding concentrations for 28 consecutive days.After treatment,serum lev-els of serum creatinine(Scr),blood urea nitrogen(BUN),interleukin-4(IL-4),and interleukin-13(IL-13)were measured.Histopathological changes in the kidney tissues were observed using hematoxylin and eosin(HE)staining and Masson's trichrome staining.Immunohistochemistry was performed to assess the expression of CD86,CD206,α-smooth muscle actin(α-SMA),and type Ⅰ collagen(Collagen Ⅰ)in renal tissues.The expression of miR-142-5p in kidney tissue was determined by qRT-PCR,and the protein expression levels of SOCS1,JAK3,and STAT6 were ana-lyzed using Western Blotting.Results:Compared with the model group,Shenshuai Xiezhuo decoction significantly re-duced serum levels of Scr,BUN,IL-4,and IL-13 in CRF rats,alleviated renal tissue injury and fibrosis,suppressed the renal expression of CD86,CD206,α-SMA,and Collagen I,downregulated the expression of miR-142-5p,upregulated SOCS1 protein levels,and downregulated the expression of JAK3 and STAT6.Conclusion:Shenshuai Xiezhuo decoc-tion may delay renal fibrosis by inhibiting the MMT process,and its mechanism may be associated with regulation of the miR-142-5p/SOCS1/JAK3 signaling axis.关键词
肾纤维化/肾衰泄浊汤/巨噬细胞向肌成纤维细胞转换/miR-142-5p/JAK3/STAT6 信号通路/炎纤转化Key words
Renal fibrosis/Shenshuai Xiezhuo decoction/Macrophage-to-myofibroblast transition/miR-142-5p/JAK3/STAT6 signaling pathway/Inflammation-fibrosis transformation分类
医药卫生引用本文复制引用
罗玉芳,杨军平,李怀玉,吕森浩,李玮婷..肾衰泄浊汤对慢性肾衰大鼠肾脏巨噬细胞向肌成纤维细胞转换的影响[J].陕西中医,2025,46(12):1604-1609,6.基金项目
江西省自然科学基金资助重点项目(20232ACB206055) （20232ACB206055）
江西省教育厅科学技术研究项目(GJJ211254) （GJJ211254）
江西省中医药管理局科技计划项目(2024B0267) （2024B0267）
