陕西中医2025，Vol.46Issue(12)：1616-1621,6.DOI:10.3969/j.issn.1000-7369.2025.12.006
加味泻青丸调控VEGF和IL-18/IFN-γ对TS大鼠神经免疫炎症的作用研究
Effect of modified Xieqing pills on VEGF and IL-18/IFN-γin regulating neuroimmune inflammation in TS model rats
摘要
Abstract
Objective:To explore the therapeutic effect and potential mechanism of modified Xieqing pills on Tourette syndrome(TS)model rats.Methods:Sixty experimental rats were divided into a blank group(n=10)and an iminodipropionitrile(IDPN)modeling group(n=50),and the modeling lasted for 7 days.The post-construction model group was evenly divided into:the model group,the Tiapride group,and the low-,medium-,and high-dose modified Xieqing pills group.Rats in the six groups were given corresponding drug intragastric intervention for 14 consecutive days.After gavage,the animals were sacrificed and specimens were collected.Reverse transcription PCR was employed to measure VEGF mRNA levels in the rat striatum.Serum levels of interleukin-18(IL-18)and inter-feron-gamma(IFN-γ)were measured via ELISA,while histological assessments of brain tissue were performed using standard HE staining protocols.Results:①Administration of modified Xieqing pills at varying doses and tiapride treatment both produced significant body weight gains in treated rats relative to model controls(P<0.05).②Signifi-cant reductions in motor behavior scores were observed in rats receiving medium and high-dose traditional Chinese medicine(P<0.01),while the tiapride-treated group exhibited moderate improvement(P<0.05).Notably,the high-dose modified Xieqing pills group demonstrated the most pronounced decrease in stereotyped movements(P<0.05),with effects surpassing those of tiapride treatment.③The model group exhibited substantial reductions in stri-atal VEGF protein levels(P<0.01)alongside pronounced upregulation of IL-18 and IFN-γ(P<0.01)before treat-ment.④Post-treatment assessments demonstrated that both medium and high-dose modified Xieqing pills formula-tions,along with tiapride administration,significantly upregulated striatal VEGF expression(P<0.01)while concur-rently suppressing IL-18 and IFN-γ production(P<0.01).⑤In the groups treated with high-dose and low-dose modified Xieqing pills,there were more remarkable reductions in IL-18 and IFN-γas well as a more notable increase in VEGF in the group of high-dose,and the differences were statistically significant(P<0.05).Conclusion:Modified Xieqing pills have a significant improvement effect on the behavioral symptoms of TS rats.The mechanism may be related to promoting the expression of VEGF in the brain tissue of TS rats,down-regulating the levels of IFN-γand IL-18,inhibiting neuroimmune inflammation,and protecting the nerves.关键词
抽动秽语综合征/加味泻青丸/血管内皮生长因子/神经免疫炎症/白介素-18/干扰素-γKey words
Tourette syndrome/Modified Xieqing pills/Vascular endothelial growth factor/Neuroimmune in-flammation/Interleukin-18/Interferon-γ分类
医药卫生引用本文复制引用
张舒婧,周姗,姚冰,周霞,谢静..加味泻青丸调控VEGF和IL-18/IFN-γ对TS大鼠神经免疫炎症的作用研究[J].陕西中医,2025,46(12):1616-1621,6.基金项目
湖南省自然科学基金资助项目(2023JJ60344) （2023JJ60344）
湖南省卫生健康委员会课题(202206015012) （202206015012）
