上海农业学报2025，Vol.41Issue(6)：84-92,9.DOI:10.15955/j.issn1000-3924.2025.06.10
不同来源曲霉菌在谷物中黄曲霉毒素的发生规律研究
Occurrence pattern of aflatoxins in grains by Aspergillus spp.from different sources
摘要
Abstract
To investigate the occurrence pattern of aflatoxins produced by different Aspergillus species,the toxin-producing conditions,amounts,and types of aflatoxins produced by Aspergillus fllavus,Aspergillus parasiticus,Aspergillus oryzae,Aspergillus pseudoglaucus and Aspergillus jensenii isolated from substrates such as grains,feeds and soils were examined in this study.The content of aflatoxins in maize and wheat cultures were determined using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The results showed that the dominant aflatoxin-producing strain was Aspergillus fllavus,with a total of 28 strains(71.8％),and the maximum production of aflatoxin B1(AFB1)reached 530.73 mg/kg.Aspergillus flavus,Aspergillus oryzae,and Aspergillus parasiticus could produce AFB1,aflatoxin B2(AFB2),aflatoxin G1(AFG1),and aflatoxin G2(AFG2)at the same time,with the maximum toxin production of 111.36,21.86,46.79,12.70 mg/kg,respectively.Aspergillus jensenii was able to produce aflatoxin M1(AFM1),but its highest production was only 1％of AFB1.The types of aflatoxins produced by different strains in maize and wheat cultures were generally consistent,which all showed a trend of first increasing followed by decreasing.The peak of aflatoxins occurred at the 3 rd or 4th week,but the production of aflatoxin varied significantly.This study revealed the occurrence pattern of aflatoxins produced by different Aspergillus species in grains,providing a scientific basis for aflatoxins prevention and control,and the preparation of reference materials.关键词
曲霉菌/黄曲霉毒素/发生规律/标准物质/食品安全Key words
Aspergillus species/Aflatoxins/Occurrence pattern/Reference material/Food safety分类
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李剑池,牛雪珂,王欣艺,孟佳佳,韩铮,郭文博..不同来源曲霉菌在谷物中黄曲霉毒素的发生规律研究[J].上海农业学报,2025,41(6):84-92,9.基金项目
国家重点研发计划项目(2024YFF1105800) （2024YFF1105800）
