上海海洋大学学报2025，Vol.34Issue(6)：1203-1213,11.DOI:10.12024/jsou.20241004669
梨形环棱螺Map2k1基因的分子特征和功能分析
Molecular characteristics and functional analysis of Map2k1 in Bellamya purificata
摘要
Abstract
In order to investigate the role of Map2k1 gene in the gonadal development and sex differentiation of Bellamya purificata,gene cloning,quantitative real-time PCR(qRT-PCR),in situ hybridization,and RNA interference were employed.The results revealed that the full-length ORF of Map2k1 cDNA was 1 191 bp,encoding 396 amino acids.The MAP2K1 protein contained the S-TKc domain,and its amino acid sequence had high homology with mollusks.Map2k1 was detected in tissues of both sexes,showing the highest expression in the female gonads,where expression significantly differed from that in the male gonads(P<0.01).In situ hybridization analysis demonstrated strong hybridization signals of Map2k1 in the oocytes and ovarian vesicles of female B.purificata.The RNA interference results showed that in female B.purificata,the interference chain G1 of Map2k1 reached the highest interference rate of 73.1%12 hours after injection,and led to a significant decrease in the expression of other sex-related genes β-catenin,Cyp17a1,and Sox9.Research had shown that Map2k1 may be involved in the gonadal development and sex differentiation of female B.purificata.This study can provide theoretical reference for the gonadal development and monosex breeding of B.purificata.关键词
梨形环棱螺/Map2k1基因/性腺发育/RNA干扰Key words
Bellamya purificata/Map2k1/gonadal development/RNAi分类
农业科技引用本文复制引用
金鑫,缪煜琳,王萍,顾洋,吕伙红,汪桂玲..梨形环棱螺Map2k1基因的分子特征和功能分析[J].上海海洋大学学报,2025,34(6):1203-1213,11.基金项目
国家重点研发计划(2022YFD2400700) （2022YFD2400700）
国家自然科学基金(32573497) （32573497）
