上海海洋大学学报2025，Vol.34Issue(6)：1214-1225,12.DOI:10.12024/jsou.20241104690
上海引种美洲鲥养殖和野生群体遗传变异的微卫星分析
Microsatellite analysis on genetic variance in cultured and wild population of American shad(Alosa sapidissima)induced into Shanghai
摘要
Abstract
Alosa sapidissima has emerged as a newly imported premium fish species with high value in promoting large-scale aquaculture in China in recent years.Understanding the genetic background of cultured and wild populations of A.sapidissima is crucial for evaluating germplasm resources and breeding new strains.In this study,20 polymorphic simple sequence repeats(SSRs)were selected to evaluate the genetic diversity and genetic structure of 3 cultured populations and 2 wild populations of A.sapidissima.A total of 201 alleles were detected from 20 polymorphic microsatellite markers average of 10.05 alleles per marker.Among the five populations,the average of the effective alleles(Ne),the observed heterozygosity(HO),expected heterozygosity(He)and polymorphism information content(PIC)ranged from 2.675 4 to 4.136 0,from 0.675 0 to 0.775 0,from 0.601 8 to 0.729 8 and from 0.547 3 to 0.698 8,respectively.The analysis of molecular variance(AMOVA)indicated that the genetic variance within individuals accounts for 94.93%and the genetic variance among populations accounts for 5.07%.The result of genetic differentiation index(Fst)analysis showed that the YSF1 had a moderate level of genetic differentiation from the YZF4 and YZF5(0.05<Fst<0.15),and the genetic differentiation of the other pairwise populations was weak(Fst<0.05).The result of the Hardy-Weinberg equilibrium showed that 31%of group loci were found to deviate significantly from the Hardy-Weinberg equilibrium after sequential Bonferroni correction(P<0.05).The UPGMA cluster tree based on the Nei's genetic distance showed that five populations were divided into two branches and the similar result also obtained from PCoA analysis and Bayesian Structure clustering analysis.Results indicated that the cultured populations of A.sapidissima introduced into Shanghai retain a relatively high level of genetic variation and hold significant genetic breeding potentiality.关键词
美洲鲥/养殖群体/微卫星标记/遗传多样性/遗传分化Key words
Alosa sapidissima/cultured population/microsatellite marker/genetic diversity/genetic differentiation分类
农业科技引用本文复制引用
于爱清,施永海,徐嘉波,刘永士,杨明,蒋飞,严银龙..上海引种美洲鲥养殖和野生群体遗传变异的微卫星分析[J].上海海洋大学学报,2025,34(6):1214-1225,12.基金项目
上海市科技兴农重点攻关项目(2021-02-08-00-12-F00788) （2021-02-08-00-12-F00788）
上海领军人才项目(沪人社专2021-67号) （沪人社专2021-67号）
上海长江口主要经济水生动物人工繁育工程技术研究中心项目(13DZ2251800) （13DZ2251800）
