上海海洋大学学报2025，Vol.34Issue(6)：1240-1250,11.DOI:10.12024/jsou.20240904626
基于18S rDNA的3种投喂模式与食性分析下中华绒螯蟹扣蟹对池塘饵料生物利用的差异比较
Comparative analysis of the utilization of pond food organisms by juvenile Chinese mitten crab under three diets based on 18S rDNA and diet analysis
摘要
Abstract
To investigate the growth and feeding conditions of the juvenile Chinese mitten crab(Eriocheir sinensis)under different diets,traditional(wheat,bran,and soybean meal,Diet 1),formulated(Diet 2),and mixed feeds(1:1 mixture of traditional and formulated feeds,Diet 3)were fed in different crab ponds in the study.During the experiment,the stomach contents of juvenile crabs under the different feeding modes were collected in August.The main potential eukaryotic food components were studied using 18S rDNA sequencing.The results showed that the Chao index of food composition under Diet 1 was significantly higher than Diet 3,while the Simpson index was opposite.The phyla with the highest relative abundance in the stomach contents of juvenile crabs for Diet 1 are,in order:Phragmoplastophyta(mainly Chlorophyceae and Trebouxiophyceae),Ciliophora(mainly Intramacronucleata),and Ochrophyta(mainly Eustigmatophyceae and Chrysophyceae).Diet 2 is Phragmoplastophyta,Arthropoda(mainly Malacostraca),and Ochrophyta.Diet 3 is Phragmoplastophyta,Arthropoda,and Vertebrata(mainly Pisces).The abundance of Ascomycota in Diet 2 and 3was significantly higher than Diet 1.There was no significant difference in the food composition in the stomach contents between male and female juvenile crabs.There was a total of 207 types of food components in the stomach contents of crabs in three diets.The main components of the stomach content of juvenile crabs in Diet 2 completely encompass the main components found in other diets.In summary,the diversity and abundance of food in the stomach contents were different under different diets.The main contents of the stomach contents in the juvenile crabs were Phragmoplastophyta,which were mainly composed of Chlorophyceae and Trebouxiophyceae.There was no significant difference in the food composition in the stomach contents between male and female juvenile crabs.关键词
中华绒螯蟹扣蟹/投喂模式/胃内含物/18S rDNA/食物组成Key words
juvenile Eriocheir sinensis/diets/stomach content/18S rDNA/food composition分类
农业科技引用本文复制引用
徐广伟,鹿珍珍,孙云飞,成永旭..基于18S rDNA的3种投喂模式与食性分析下中华绒螯蟹扣蟹对池塘饵料生物利用的差异比较[J].上海海洋大学学报,2025,34(6):1240-1250,11.基金项目
国家自然科学基金青年基金(31802320) （31802320）
国家现代农业产业技术体系(CARS-48) （CARS-48）
