上海海洋大学学报2025，Vol.34Issue(6)：1263-1269,7.DOI:10.12024/jsou.20241104707
养殖水体的核酸富集技术在鳜蛙虹彩病毒检测中的应用及其性能评价
Application of nucleic acid enrichment technology in culture water for the detection of iridovirus in Mandarin fish ranavirus and evaluation of its performance
摘要
Abstract
The aim of this study was to develop a magnetic bead enrichment-based method for the detection of Mandarin fish ranavirus in culture water.The results showed that the method was effective in enriching Mandarin fish ranavirus in culture water samples,and specific bands could be obtained by conventional PCR amplification.Quantitative analysis showed that the sensitivity of the magnetic bead enrichment method combined with real-time fluorescence quantitative PCR reached the detection limit of 0.01 copies/μL.Feasibility analysis demonstrated that the magnetic bead enrichment method had 90%concordance with qPCR results and was suitable for fully automated nucleic acid detection platforms.Compared with the traditional qPCR method,this method has better anti-interference ability and ease of operation while maintaining high detection consistency.The detection technology developed in this study is characterized by easy operation and high sensitivity,which provides reliable technical support for the monitoring of aquaculture water pathogens and early warning,and is of great practical significance for the prevention and control of aquaculture diseases.关键词
鳜蛙虹彩病毒/养殖水体/病毒检测/磁珠法Key words
Mandarin fish ranavirus/aquaculture water body/virus detection/magnetic bead method分类
农业科技引用本文复制引用
许童辉,任馨雨,任弋林,张俊哲,翁少萍,许丹,王姝然..养殖水体的核酸富集技术在鳜蛙虹彩病毒检测中的应用及其性能评价[J].上海海洋大学学报,2025,34(6):1263-1269,7.基金项目
水产动物疫病防控与健康养殖全国重点实验室2024年开放课题(2024SKLBC-KF02) （2024SKLBC-KF02）
上海市自然科学基金面上基金(22ZR1427200) （22ZR1427200）
