上海海洋大学学报2025，Vol.34Issue(6)：1281-1291,11.DOI:10.12024/jsou.20250304805
鳗弧菌侵染对菲律宾蛤仔存活、鳃组织及免疫基因表达的影响
Effects of Vibrio anguillarum infection on the survival,gill tissue and immune-related gene expression of Ruditapes philippinarum
摘要
Abstract
To explore the semi-lethal concentration(LC50)of Vibrio anguillarum infection in Ruditapes philippinarum and its effects on gill tissues and immune related genes.In this study,R.philippinarum was challenged with five different concentrations of V.anguillarum for 168 h.Meanwhile,the LZM activity,bacterial load,pathological analysis and the expression of immune-related genes in the gills were conducted.The results showed that the challenge group injected with V.anguillarum at 5×10⁷ CFU/mL for 168 h corresponded to the semi-lethal concentration(LC50)group,and the semi-lethal concentration(LC50)was 6.08×106 CFU/(mL·g),with a survival rate of 52.3%.The survival rate was the lowest at 33.6%in the 5×108 CFU/mL group and the highest survival rate of 67.0%was observed in the 5×106 CFU/mL group.The results indicated that the bacterial load peaked at 24 h(7.53 CFU/mg),and then gradually decreased,reaching 4.26 CFU/mg at 120 h in semi-lethal concentration(LC50)groups.The LZM activity significantly increased in the gills and peaked at 12 h(123.03 U/mg prot/mL),and then gradually decreased,reaching 77.92 U/mg prot/mL at 120 h.The pathological analysis revealed that the gills filaments showed disordered arrangement,severe shrinkage,and increased gaps at 24 h.Additionally,quantitative real-time PCR(qPCR)analysis revealed that V.anguillarum infection significantly upregulated the expression of several immune related genes,including heat shock protein(Hsp70),Cytochrome p450(Cyp1a1),serine/threonine protein kinase pim3(Pim3),Lysozyme,Leukocyte differentiation antigen(Cd109)and tumor necrosis factor receptor associated factor 6(Traf6)(P<0.05),revealing their important roles in the immune defense response of R.philippinarum against V.anguillarum.This study preliminarily explored the effects of V.anguillarum on R.philippinarum,providing new insights for the innate immune mechanism of the R.philippinarum against V.anguillarum.关键词
菲律宾蛤仔/鳗弧菌/鳃组织/半致死浓度Key words
Ruditapes philippinarum/Vibrio anguillarum/gill/half-lethal concentration(LC50)分类
农业科技引用本文复制引用
贾建鑫,王瑞瑶,李兵辉,聂鸿涛..鳗弧菌侵染对菲律宾蛤仔存活、鳃组织及免疫基因表达的影响[J].上海海洋大学学报,2025,34(6):1281-1291,11.基金项目
辽宁省兴辽英才青年拔尖人才项目(XLYC2403165) （XLYC2403165）
辽宁省教育厅基本科研项目(LJ212410158027) （LJ212410158027）
大连市杰出青年项目(2024RJ014) （2024RJ014）
