世界中医药2025，Vol.20Issue(15)：2619-2625,7.DOI:10.3969/j.issn.1673-7202.2025.15.004
基于生信分析探讨金雀异黄素对非小细胞肺癌的影响
Effect of Genistein on Non-small Cell Lung Cancer Based on Bioinformatics Analysis
摘要
Abstract
Objective:To investigate the effect of genistein on non-small cell lung cancer(NSCLC)A549 cells and its potential therapeutic targets through bioinformatics analysis and cell biology experiments.Methods:A549 cells were divided into a control group and a genistein treatment group(100 μmol/L).After 48 hours of treatment,RNA was extracted using an RNA extraction kit and subjected to transcriptome sequencing.Differentially expressed genes were analyzed using heatmaps and volcano plots on the Sangerbox website.Gene Ontology(GO)enrichment analysis was performed using the Metascape website.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis was conducted using OmicShare.Drug-disease intersecting targets were uploaded to Cytoscape v3.7.2 for protein-protein interaction(PPI)analysis.Flow cytometry was used to validate the effect of genistein on the cell cycle of A549 cells.Results:Compared with the control group,genistein treatment altered mRNA expression,with significant differences between the two groups.A total of 4 630 differentially expressed genes were identified,including 2 853 upregulated genes and 1 777 downregulated genes.KEGG enrichment analysis indicated that genistein affected the cell cycle.Flow cytometry re-sults demonstrated that genistein induced G2/M phase arrest in A549 cells and simultaneously affected mitotic progression.Conclu-sion:Genistein may inhibit NSCLC progression by influencing the A549 cell cycle,DNA replication,and p53-related signaling path-ways.关键词
生物信息学/金雀异黄素/转录组测序/非小细胞肺癌/人肺癌细胞系细胞A549/流式细胞术/细胞周期/有丝分裂Key words
Bioinformatics/Genistein/Transcriptome sequencing/Non-small cell lung cancer/A549/Flow cytometry/Cell cy-cle/Mitosis分类
医药卫生引用本文复制引用
产柳佳,王阳,谢楚菲,赵立春..基于生信分析探讨金雀异黄素对非小细胞肺癌的影响[J].世界中医药,2025,20(15):2619-2625,7.基金项目
国家自然科学基金项目(82160775) （82160775）
广西自然科学基金重点项目(2021GXNSFDA075008) （2021GXNSFDA075008）
