世界中医药2025，Vol.20Issue(15)：2649-2656,8.DOI:10.3969/j.issn.1673-7202.2025.15.008
复方丹参方调控动脉粥样硬化小鼠肝脏脂质沉积的作用机制
Mechanism of Fufang Danshen Prescription in Regulating Hepatic Lipid Deposition in Atherosclerosis Mice
摘要
Abstract
Objective:To explore the mechanism by which Fufang Danshen Prescription(FFDS)regulates liver lipid deposition in ApoE-/-atherosclerosis(AS)mice based on the Nrf2/GPX4 signaling pathway.Methods:Twenty-seven ApoE-/-mice were ran-domly divided into a model group,FFDS group,and rosuvastatin group(n=9 per group)according to a random number table.Nine age-matched C57BL/6J mice were used as the normal group.All mice were fed a high-fat diet for 12 weeks.The FFDS group re-ceived FFDS(105.3 mg/kg)by gavage,the rosuvastatin group received rosuvastatin(1.3 mg/kg)by gavage,and the normal and model groups received an equal volume of saline.After 8 weeks of treatment,liver tissues were collected for Oil Red O staining,he-matoxylin-eosin(HE)staining,and Prussian blue staining to assess lipid deposition,tissue morphology,and iron accumulation.Mi-tochondrial morphology was observed by transmission electron microscopy(TEM).Serum levels of cholesterol(CHOL),triglycerides(TG),high-density lipoprotein(HDL),and low-density lipoprotein(LDL)were measured using commercial kits.Liver function in-dicators,including alanine transaminase(ALT),aspartate transaminase(AST),total bilirubin(TB),and direct bilirubin(DB),were determined.Malondialdehyde(MDA),reduced glutathione(GSH),and iron content in liver tissues were measured by kits.RT-qPCR and Western blot were used to detect mRNA and protein expression of GPX4,Nrf2,HO-1,and xCT in liver tissue.Results:Compared with the model group,FFDS treatment reduced hepatic lipid droplet size,mitigated degeneration,and significantly de-creased iron accumulation and mitochondrial damage.Serum lipid levels were significantly reduced(P＜0.05,P＜0.01).Liver MDA and iron content were significantly decreased(P＜0.01),while GSH content was significantly increased(P＜0.01).Moreo-ver,mRNA and protein expression levels of GPX4,Nrf2,HO-1,and xCT were significantly upregulated(P＜0.05,P＜0.01).Con-clusion:FFDS inhibits ferroptosis in the liver of ApoE-/-AS mice and reduces liver lipid deposition,potentially through activation of the Nrf2/GPX4 signaling pathway.关键词
复方丹参方/铁死亡/肝脏脂质沉积/动脉粥样硬化/Nrf2/GPX4信号通路/激活Key words
Fufang Dansheng Prescription/Ferroptosis/Liver lipid deposition/Atherosclerosis/Nrf2/GPX4 signaling pathway/Activation分类
医药卫生引用本文复制引用
杨琼,刘德坤,宋月月,贾雨涵,杨雯晴,张丹..复方丹参方调控动脉粥样硬化小鼠肝脏脂质沉积的作用机制[J].世界中医药,2025,20(15):2649-2656,8.基金项目
国家自然科学基金项目(82174337) （82174337）
山东省自然科学基金项目(ZR202212050026) （ZR202212050026）
山东省高等学校"青创团队计划"(2022KJ257) （2022KJ257）
