世界中医药2025，Vol.20Issue(15)：2657-2661,5.DOI:10.3969/j.issn.1673-7202.2025.15.009
黄芪甲苷调控多囊卵巢综合征大鼠颗粒细胞自噬和凋亡的作用机制研究
Mechanism of Astragaloside Ⅳ in Regulating Autophagy and Apoptosis of Granulosa Cells in Rats with Polycystic Ovary Syndrome
摘要
Abstract
Objective:To investigate the effects of astragaloside Ⅳ(AST Ⅳ)on autophagy and apoptosis of granulosa cells in polycystic ovary syndrome(PCOS)rats by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammali-an target of rapamycin(mTOR)signaling pathway.Methods:A PCOS rat model was established,and granulosa cells were collected from both normal and PCOS rats.Granulosa cells isolated from normal rats served as the control group.Granulosa cells from PCOS rats were divided into the model group,AST Ⅳ group(20 μ-g/mL AST IV),activator group(100 μmol/L 740Y-P,a PI3K/AKT/mTOR pathway activator),and AST Ⅳ+activator group(20 μg/mL AST Ⅳ+100 μmol/L 740Y-P).Cell viability and cytotoxicity were assessed using the MTT assay.Apoptosis of granulosa cells was measured by flow cytometry.Autophagosomes were observed by transmission electron microscopy(TEM).Expression of autophagy-related proteins and PI3K/AKT/mTOR pathway proteins was an-alyzed by Western blot.Results:Compared with the control group,the model group showed increased optical density(OD)at 570 nm,elevated levels of phosphorylated(p)-PI3K/PI3K,p-AKT/AKT,and mTOR proteins(P＜0.05),and decreased apoptosis rate,Beclin1,LC3-Ⅱ/Ⅰ protein levels,and autophagosome number(P＜0.05).Compared with the model group,the AST Ⅳ group exhibited decreased OD570,p-PI3 K/PI3 K,p-AKT/AKT,and mTOR protein levels(P＜0.05),along with increased apoptosis rate,Beclinl,LC3-Ⅱ/Ⅰ protein levels,and autophagosome number(P＜0.05),whereas the activator group showed opposite trends.Moreover,740Y-P attenuated the effects of AST Ⅳ on autophagy and apoptosis of granulosa cells in PCOS rats.Conclusion:AST IV may promote autophagy and apoptosis of granulosa cells in PCOS rats by inhibiting the PI3K/AKT/mTOR signaling pathway.关键词
黄芪甲苷/多囊卵巢综合征/PI3K/AKT/mTOR信号通路/颗粒细胞/凋亡/自噬/SD大鼠/增殖Key words
Astragaloside Ⅳ/Polycystic ovary syndrome/PI3 K/AKT/mTOR signaling pathway/Granulosa cells/Apoptosis/Au-tophagy/SD rat/Proliferation分类
医药卫生引用本文复制引用
吴晓春,黄燕,周玉珍,陈惠娴,申小娟,赵轩懿..黄芪甲苷调控多囊卵巢综合征大鼠颗粒细胞自噬和凋亡的作用机制研究[J].世界中医药,2025,20(15):2657-2661,5.基金项目
国家自然科学基金项目(81902911) （81902911）
