世界中医药2025，Vol.20Issue(15)：2662-2670,9.DOI:10.3969/j.issn.1673-7202.2025.15.010
清胃泄热方对肥胖大鼠肠道菌群的影响
Effect of Qingwei Xiere Formula on Intestinal Flora of Obese Rats
摘要
Abstract
Objective:To investigate the effect of Qingwei Xiere Formula on the intestinal flora of obese rats.Methods:Fifty-six rats were randomly assigned to the blank group,model group,Orlistat group(32.1 mg/kg),and Qingwei Xiere Formula low-and high-dose groups(222 and 444 mg/kg),with 8 rats per group.Except for the blank group,all other groups were fed a high-fat diet to establish an obesity model.Body weight was recorded,and blood lipid and liver function indices were measured.Liver histology was observed,and the types and abundance of intestinal flora in fecal samples were analyzed,followed by functional prediction.Re-sults:Compared with the model group,rats in the high-dose Qingwei Xiere Formula group showed decreased body weight and im-proved blood lipid and liver function indices(P＜0.05).Liver tissue damage was alleviated,and the degree of hepatocyte steatosis was reduced.Differences in intestinal flora abundance were observed before and after Qingwei Xiere Formula intervention.At the phylum level,compared with the model group,the abundance of Firmicutes decreased in both low-and high-dose Qingwei Xiere For-mula groups(P＜0.01),while the abundance of Verrucomicrobia and Bacteroidetes increased(P＜0.01,P＞0.05).At the genus level,the abundance of Lactobacillus and Bacteroides increased(P＜0.01,P＜0.05),whereas the abundance of Ruminococcus de-creased(P＜0.01).Functional pathway prediction of differential species among groups indicated that the most abundant metabolic pathways were mainly related to carbohydrate metabolism,amino acid metabolism,and energy metabolism.Conclusion:Qingwei Xiere Formula can reduce body weight and blood lipid levels in obese rats,alleviate hepatic steatosis and liver injury,and its effects may be associated with modulation of the intestinal flora.关键词
清胃泄热方/肥胖/肠道菌群/大鼠/肝脂肪变性/肝损伤/物种丰度/代谢途径Key words
Qingwei Xiere Formula/Obesity/Intestinal flora/Rats/Hepatic steatosis/Hepatic injury/Species abundance/Met-abolic pathways分类
医药卫生引用本文复制引用
李若楠,王萍,王晨晓,刘斌,金小琴,燕树勋..清胃泄热方对肥胖大鼠肠道菌群的影响[J].世界中医药,2025,20(15):2662-2670,9.基金项目
国家中医临床研究基地科研专项(2021JDZY034) （2021JDZY034）
河南省中医药科学研究专项课题(2022ZY1030) （2022ZY1030）
河南省高等学校重点科研项目(23A360006,24B360001) （23A360006,24B360001）
