银鲫不同克隆系疱疹病毒抗性差异及全基因组关联分析
RESISTANCE DIFFERENCES TO CARASSIUS AURSTUS HERPESVIRUS AND THE GENOME-WIDE ASSOCIATION STUDY AMONG DIFFERENT CLONES OF GIBEL CARP
摘要
Abstract
To investigate the resistance differences against Carassius auratus herpesvirus(CaHV)among different gynogenetic clones of gibel carp(Carassius gibelio)and identify potential antiviral resistance-related genes,seven gynogenetic clones and performed microsatellite identification on their maternal individuals.These seven clones were established,including three Fangzheng clones(FZ1,FZ2,and FZ3),one Poyang clone(PY),one Pengze clone(PZ),along with two control clones:the highly resistant clone H and the susceptible variety allogynogenetic gibel carp"CASⅢ"(clone A+).Subsequently,artificial infection experiments with CaHV were conducted on these clones.The survival rates,ranked from highest to lowest,were:H(100%)>FZ2(95%)>PY(40%)>FZ1(20%)=FZ3(20%)=PZ(20%)>A+(10%).At 48h post-infection,histological observations revealed that the liver cells of the highly resistant clones FZ2 and H exhibited tighter cellular arrangements and significantly lower expression levels of CaHV gene orf72,indicating low viral load.In contrast,susceptible clones FZ1,FZ3,PZ,and A+displayed swollen liver cells and elevated orf72 expression,suggesting high viral load.Additionally,whole-genome resequencing of 10 offspring from each clone iden-tified 35278852 single nucleotide polymorphism loci.Genome-wide association study mapped 63 genomic regions associated with disease resistance traits,encompassing 213 annotated genes.Integrative analysis with transcriptomic data and qPCR validation identified 11 potential antiviral resistance-related genes.This study not only identifies supe-rior germplasm with high resistance to CaHV but also screens potential genes for disease resistance,laying a founda-tion for antiviral disease-resistant breeding in gibel carp.关键词
雌核生殖/克隆系/鲫疱疹病毒/抗病育种/银鲫Key words
Gynogenesis/Clone/CaHV/Disease resistant breeding/Gibel carp分类
农业科技引用本文复制引用
刘雪倩,周莉,李顺,桂建芳,李熙银,李卓聪,王忠卫,田君,李志,丁苗,何洁,雷瑞彬,朱子彧..银鲫不同克隆系疱疹病毒抗性差异及全基因组关联分析[J].水生生物学报,2025,49(12):49-63,15.基金项目
国家重点研发计划(2021YFD1200804) （2021YFD1200804）
中国科学院先导专项(XDB0730300) （XDB0730300）
国家自然科学基金(U23A20245) （U23A20245）
国家大宗淡水鱼产业技术体系(CARS-45-02) （CARS-45-02）
湖北省科技重大专项(2023BBA001) （2023BBA001）
湖北省青年拔尖人才项目(李熙银,2023) （李熙银,2023）
武汉市自然科学基金(2024040701010070) （2024040701010070）
水产品种创制与高效养殖全国重点实验室专项基金(2024BBSA02)资助[Supported by the National Key R&D Program of China(2021YFD1200804) （2024BBSA02）
the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0730300) （XDB0730300）
the National Natural Science Foundation of China(U23A20245) （U23A20245）
the Earmarked Fund for China Agricul-ture Research System(CARS-45-02) （CARS-45-02）
the Science and Technology Major Project in Hubei Province(2023BBA001) （2023BBA001）
the Youth Talent Support Program of Hubei Province(2023,Xi-Yin Li) （2023,Xi-Yin Li）
the Natural Science Foundation of Wuhan(2024040701010070) （2024040701010070）
the Special Funds for State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture(2024BBSA02)] （2024BBSA02）
