水生生物学报2025，Vol.49Issue(12)：83-94,12.DOI:10.3724/1000-3207.2025.2025.0092
β-胡萝卜素羟化酶调控衣藻光保护与鞭毛协调性摆动的分子机制
MOLECULAR MECHANISM OF β-CAROTENE HYDROXYLASE IN REGULA-TION OF PHOTOPROTECTION AND COORDINATED FLAGELLAR BEATING IN CHLAMYDOMONAS REINHARDTII
摘要
Abstract
To investigate the function and mechanism of β-carotene hydroxylase(CHYB)in Chlamydomonas rein-hardtii,CRISPR/Cas9-mediated gene editing was used to precisely knockout CHYB gene from Chlamydomonas rein-hardtii and successfully isolated the chyb mutants.Phenotypic analysis revealed that CHYB knockout significantly decreased photosynthetic activity of photosystem Ⅱ(PSⅡ),light utilization efficiency(Alpha),and photosynthetic electron transport rate(ETRmax)in chyb mutants,ultimately reducing cell growth rate.Under high light conditions,the photoprotection related non-photochemical quenching ability of chyb mutants was inhibited.Targeted metabolomics analysis revealed the contents of zeaxanthin,antheraxanthin,and violaxanthin,which were the key components of the xanthophyll cycle,were significantly reduced in chyb mutants,thereby affecting the photoprotection ability of Chlamy-domonas reinhardtii.In addition,mutations in the CHYB gene were found to affect flagellar beating coordination,resulting in the inability of Chlamydomonas reinhardtii to sustain linear movement.These findings establish dual phy-siological roles of CHYB in Chlamydomonas reinhardtii:(1)maintaining the non-photochemical quenching ability of Chlamydomonas reinhardtii by regulating the synthesis of key components of xanthophyll cycle;(2)potentially regu-lating flagellar beating coordination via carotenoid synthesis in eyespots.These findings provide novel insights into the role of carotenoids in regulating the environmental adaptability of photosynthetic organisms.关键词
β-胡萝卜素羟化酶/类胡萝卜素代谢/光合作用/鞭毛协调性摆动/莱茵衣藻Key words
β-carotene hydroxylase/Carotenoid metabolism/Photosynthesis/Coordinated flagellar beating/Chlamy-domonas reinhardtii分类
生物科学引用本文复制引用
袁筱玉,刘盖,邓璇,黄开耀..β-胡萝卜素羟化酶调控衣藻光保护与鞭毛协调性摆动的分子机制[J].水生生物学报,2025,49(12):83-94,12.基金项目
国家自然科学基金(32270744)资助[Supported by the National Natural Science Foundation of China(32270744)] （32270744）
