水生生物学报2025，Vol.49Issue(12)：95-104,10.DOI:10.3724/1000-3207.2025.2025.0133
希瓦氏菌发酵棉籽蛋白替代鱼粉对大菱鲆生长及非特异性免疫的影响
SHEWANELLA SP.FERMENTED COTTONSEED PROTEIN REPLACING FISH-MEAL ON GROWTH AND NON-SPECIFIC IMMUNITY OF TURBOT(SCOPH-THALMUS MAXIMUS L.)
摘要
Abstract
In order to investigate the effect of Shewanella sp.fermented cottonseed protein replacing fishmeal on growth performance and non-specific immunity of turbot(Scophthalmus maximus L.),this study was conducted using 50%fish meal group as the control group,and experimental groups with fermented cottonseed protein and cottonseed protein were used as the experimental group replacing 20%of fish meal in the feed.Three kinds of compound feeds with isonitrogenous and isofat(fish meal group,cottonseed protein group,fermented cottonseed protein group)were prepared.Turbot with an initial body weight of(17.52±0.02)g were cultured for 8 weeks.The results showed that the weight gain rate(WGR),specific growth rate(SGR),and protein efficiency(PER)in the fermented cottonseed protein group were significantly higher than those in the cottonseed protein group,and the feed coefficient(FCR)was signifi-cantly lower(P<0.05),with no significant difference from the fish meal group.The antioxidant indices revealed that the fermented group had significantly higher total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity in the liver than that in the cottonseed protein group,significantly higher catalase(CAT)activity than that in the fish meal group,and significantly lower malondialdehyde(MAD)content than that in the fish meal group(P<0.05).Histo-morphology analysis showed that the intestinal villi height in the fermented group was significantly higher compared to the cottonseed protein group(P<0.05)and not significantly different from the fish meal group.In terms of immunity,the expression of intestinal pro-inflammatory factor genes(il-8,ifnγ,il-1β)and TLR2/NF-κB pathway genes(tlr2,myd88,nf-κb p65)was significantly suppressed in the fermented cottonseed protein group compared with the cotton-seed protein group(P<0.05),with no significant difference from the fish meal group.Conversely,the expression of tight junction protein genes(zo-1,occludin,tricelluin)was significantly up-regulated(P<0.05),in which zo-1 and occludin expression was significantly higher than that of fish meal group.Under the present experimental conditions,Shewanella sp.fermentation of cottonseed protein significantly improved the negative effects of cottonseed protein replacing fish meal on the turbot growth and immunity,and could replace 20%of fish meal in turbot feed.关键词
发酵棉籽蛋白/生长/非特异性免疫/大菱鲆Key words
Fermented cottonseed protein/Growth/Non-specific immunity/Turbot分类
农业科技引用本文复制引用
赵天豪,周慧慧,何艮,王旋,刘成栋,麦康森..希瓦氏菌发酵棉籽蛋白替代鱼粉对大菱鲆生长及非特异性免疫的影响[J].水生生物学报,2025,49(12):95-104,10.基金项目
国家重点研发计划(2023YFD2400601) （2023YFD2400601）
国家海水鱼产业技术体系项目(CARS-47-G10)资助[Supported by the National Key Research and Development Program of China(2023YFD2400601) （CARS-47-G10）
the China Agriculture Research System for Marine Fish Culture Industry(CARS-47-G10)] （CARS-47-G10）
