生物技术通报2025，Vol.41Issue(11)：47-61,15.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0713
大肠杆菌Nissle 1917的合成生物学平台开发及应用进展
Advances in Synthetic Biology Platform Development and Application for Escherichia coli Nissle 1917
摘要
Abstract
As a probiotic with broad application value across multiple fields,Escherichia coli Nissle 1917(EcN)is recognized as an ideal engineered chassis microbial platform,owing to its clinically validated safety,superior gut colonization capacity,and genetic compatibility with model strains.Consequently,the development of a mature genetic manipulation system for EcN is fundamental to achieving its functional applications in diverse areas.This article systematically reviews the probiotic properties of EcN,with a focus on summarizing recent advances in the development of genetic editing tools for this strain.It further sorts applications of genetically engineered EcN in therapeutic drug delivery systems for diseases(such as inflammatory bowel disease and tumor)and in efficient biosynthesis.Finally,the review explores the future directions and potential of engineered EcN strains,empowered by cutting-edge technologies like artificial intelligence,in the development of precise and safe gene editing tools,live biotherapeutics,and green biomanufacturing.This work aims to promote the widespread application of the EcN platform across various fields and provide insights and references for future research and industrial translation.关键词
大肠杆菌Nissle 1917/益生菌/调控元件/疾病治疗/生物合成Key words
Escherichia coli Nissle 1917/probiotics/regulatory elements/treatment of disease/synthetic biology引用本文复制引用
周思延,丁炜权,董平,翁含之,胥睿睿,康振..大肠杆菌Nissle 1917的合成生物学平台开发及应用进展[J].生物技术通报,2025,41(11):47-61,15.基金项目
国家自然科学基金项目(U24A20368,32370066,32400056),江苏省合成生物基础研究中心(BK20233003),广东省重点领域研发计划(2024B1111160002) （U24A20368,32370066,32400056）
