生物技术通报2025，Vol.41Issue(11)：62-74,13.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0032
维生素B12生物合成领域的十年回顾与技术进展
A Decade Review and Technological Advances in the Field of Vitamin B12 Biosysthesis
摘要
Abstract
Vitamin B12 is an essential small molecule compound for humans,animals and many microorganisms,which is widely involved in physiological processes such as one-carbon metabolism,homocysteine methylation and fatty acid metabolism.In recent years,with the development of microbiology and metabolic engineering,the research on microbial fermentation synthesis of vitamin B12 has made remarkable progress.In this paper,we first briefly introduce the vitamin B12 synthesis pathway,and then review several major microorganisms synthesizing vitamin B12 in the last decade,including the industrial production strain Pseudomonas denitrificans,Sinorhizobium meliloti,Ensifer adhaerens,and Propionibacterium freudenreichii,and newly emerged artificial strains in recent years for heterologous synthesis of vitamin B12.The article discusses the metabolic engineering strategies,fermentation processes and the potential application of these microorganisms in industrial production.In addition,current research challenges and future directions are analyzed,such as the application of genetic engineering modifications and metabolic regulation strategies in enhancing vitamin B12 production.The in-depth understanding of the synthetic pathways of these microorganisms is expected to provide new theoretical basis and technical support for industrial-scale vitamin B12 production.关键词
维生素B12/微生物/代谢工程/生物合成途径/工业生产菌株Key words
vitamin B12/microorganisms/metabolic engineering/biosynthetic pathways/industrial production strains引用本文复制引用
张纪娇,王会颖,房欢,张大伟..维生素B12生物合成领域的十年回顾与技术进展[J].生物技术通报,2025,41(11):62-74,13.基金项目
中国科学院战略性先导科技专项(XDC0110200),国家自然科学基金项目(22178372) （XDC0110200）
